Differential effects of dexamethasone and rosiglitazone in a sephadex-induced model of lung inflammation in rats

Possible role of tissue inhibitor of metalloproteinase-3

Jignesh K. Nagar, Praful P. Patel, Jogeswar N. Mohapatra, Manoranjan M. Sharma, Gaurav M. Pandya, Malik M. Umar, Abhijit A. Chatterjee, Shrikalp S. Deshpande, Mukul R. Jain, Hiteshkumar Soni

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objectives: To study the effects of two different classes of drugs in sephadex-induced lung inflammation using rats and explore the potential mechanism (s). Materials and Methods: Effects of dexamethasone (0.3 mg/kg, p.o.) and rosiglitazone (10 mg/kg, p.o.) treatments were evaluated up to 3 days in sephadex challenged rats. 72 h postsephadex administration, broncho-alveolar lavage fluid (BALF) was collected for cell count and cytokine estimation. Lung tissues were harvested for gene expression and histopathology. Results: Dexamethasone treatment resulted in significant inhibition of lymphocytes, monocytes, eosinophils and neutrophils, whereas rosiglitazone inhibited eosinophils and neutrophils only. Further, dexamethasone reduced the elevated levels of prostaglandin E2 (PGE2) and leukotriene B4 (LTB4) after sephadex challenge while rosiglitazone significantly reduced the PGE2 levels without altering LTB4 in the BALF. Hydroxyproline content in rat lung homogenate was significantly reduced with dexamethasone treatment but not with rosiglitazone. Both the drugs were found to suppress matrix metallo proteinase 9, whereas only dexamethasone showed inhibition of tumor necrosis factor-alpha and up-regulation of tissue inhibitor of metalloproteinase 3 (TIMP-3) expression and preserved the broncho-alveolar microstructure. Conclusions: Our results revealed that up-regulation of TIMP-3 corroborated well with dexamethasone mediated inhibition of collagen degradation and restoration of alveolar micro-architecture.

Original languageEnglish (US)
Pages (from-to)153-159
Number of pages7
JournalIndian Journal of Pharmacology
Volume47
Issue number2
DOIs
StatePublished - Mar 1 2015

Fingerprint

rosiglitazone
Tissue Inhibitor of Metalloproteinase-3
Dexamethasone
Pneumonia
Leukotriene B4
Bronchoalveolar Lavage Fluid
Dinoprostone
Eosinophils
Neutrophils
Up-Regulation
Lung
Hydroxyproline
Pharmaceutical Preparations
sephadex
Monocytes
Peptide Hydrolases
Collagen
Therapeutics
Tumor Necrosis Factor-alpha
Cell Count

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)

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Differential effects of dexamethasone and rosiglitazone in a sephadex-induced model of lung inflammation in rats : Possible role of tissue inhibitor of metalloproteinase-3. / Nagar, Jignesh K.; Patel, Praful P.; Mohapatra, Jogeswar N.; Sharma, Manoranjan M.; Pandya, Gaurav M.; Umar, Malik M.; Chatterjee, Abhijit A.; Deshpande, Shrikalp S.; Jain, Mukul R.; Soni, Hiteshkumar.

In: Indian Journal of Pharmacology, Vol. 47, No. 2, 01.03.2015, p. 153-159.

Research output: Contribution to journalArticle

Nagar, Jignesh K. ; Patel, Praful P. ; Mohapatra, Jogeswar N. ; Sharma, Manoranjan M. ; Pandya, Gaurav M. ; Umar, Malik M. ; Chatterjee, Abhijit A. ; Deshpande, Shrikalp S. ; Jain, Mukul R. ; Soni, Hiteshkumar. / Differential effects of dexamethasone and rosiglitazone in a sephadex-induced model of lung inflammation in rats : Possible role of tissue inhibitor of metalloproteinase-3. In: Indian Journal of Pharmacology. 2015 ; Vol. 47, No. 2. pp. 153-159.
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AU - Sharma, Manoranjan M.

AU - Pandya, Gaurav M.

AU - Umar, Malik M.

AU - Chatterjee, Abhijit A.

AU - Deshpande, Shrikalp S.

AU - Jain, Mukul R.

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