Differential regulation of interleukin-1α and interleukin-1β in K-562 cells

Albert T. Ichiki, Mark Langenberg, Erich J. Baker, James W. Hodge, Elena G. Bamberger, David Gerard, Carmen B. Lozzio

Research output: Contribution to journalArticle

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Abstract

Interleukin (IL)-1α and IL-1β are encoded by two separate genes, but both function as comitogens for lymphocyte activation. In this study, we observed K-562 cells to express constitutively mRNA for IL-1α, although IL- 1α was not detected in the growth-conditioned medium (GCM). However, IL-1β mRNA was not expressed unless the cells had been treated with phorbol myristate acetate (PMA). Both IL-1α and IL-1β were detected in the GCM after the cells had been cultured with PMA, suggesting that IL-1 elaboration required PMA treatment. The K-562 cells treated with PMA differentiated to the myeloblastic stage, as observed by nuclear morphologic properties by electron microscopy. PMA treatment induced de novo expression of CD61 or gpIIIa, a marker associated with megakaryoblasts. These results showed that although K-562 cells constitutively expressed IL-1α mRNA, PMA treatment was required for secretion. On the other hand, both the expression and secretion of IL-1β required treatment with PMA. This study showed that K-562 cells treated with PMA differentiated to the myeloblastic stage and expressed and secreted IL-1α and IL-1β.

Original languageEnglish (US)
Pages (from-to)1045-1050
Number of pages6
JournalJournal of Interferon and Cytokine Research
Volume18
Issue number12
DOIs
StatePublished - Jan 1 1998

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Interleukin-1
Tetradecanoylphorbol Acetate
Conditioned Culture Medium
Messenger RNA
Megakaryocyte Progenitor Cells
Lymphocyte Activation
Growth
Electron Microscopy

All Science Journal Classification (ASJC) codes

  • Immunology
  • Cell Biology
  • Virology

Cite this

Ichiki, A. T., Langenberg, M., Baker, E. J., Hodge, J. W., Bamberger, E. G., Gerard, D., & Lozzio, C. B. (1998). Differential regulation of interleukin-1α and interleukin-1β in K-562 cells. Journal of Interferon and Cytokine Research, 18(12), 1045-1050. https://doi.org/10.1089/jir.1998.18.1045

Differential regulation of interleukin-1α and interleukin-1β in K-562 cells. / Ichiki, Albert T.; Langenberg, Mark; Baker, Erich J.; Hodge, James W.; Bamberger, Elena G.; Gerard, David; Lozzio, Carmen B.

In: Journal of Interferon and Cytokine Research, Vol. 18, No. 12, 01.01.1998, p. 1045-1050.

Research output: Contribution to journalArticle

Ichiki, AT, Langenberg, M, Baker, EJ, Hodge, JW, Bamberger, EG, Gerard, D & Lozzio, CB 1998, 'Differential regulation of interleukin-1α and interleukin-1β in K-562 cells', Journal of Interferon and Cytokine Research, vol. 18, no. 12, pp. 1045-1050. https://doi.org/10.1089/jir.1998.18.1045
Ichiki, Albert T. ; Langenberg, Mark ; Baker, Erich J. ; Hodge, James W. ; Bamberger, Elena G. ; Gerard, David ; Lozzio, Carmen B. / Differential regulation of interleukin-1α and interleukin-1β in K-562 cells. In: Journal of Interferon and Cytokine Research. 1998 ; Vol. 18, No. 12. pp. 1045-1050.
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