Differential response of C57BL/6J mouse and DBA/2J mouse to optic nerve crush

Justin P. Templeton, Mohamed Nassr, Felix Vazquez-Chona, Natalie E. Freeman-Anderson, William E. Orr, Robert Williams, Eldon E. Geisert

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Background: Retinal ganglion cell (RGC) death is the final consequence of many blinding diseases, where there is considerable variation in the time course and severity of RGC loss. Indeed, this process appears to be influenced by a wide variety of genetic and environmental factors. In this study we explored the genetic basis for differences in ganglion cell death in two inbred strains of mice. Results: We found that RGCs are more susceptible to death following optic nerve crush in C57BL/6J mice (54% survival) than in DBA/2J mice (62% survival). Using the Illumina Mouse-6 microarray, we identified 1,580 genes with significant change in expression following optic nerve crush in these two strains of mice. Our analysis of the changes occurring after optic nerve crush demonstrated that the greatest amount of change (44% of the variance) was due to the injury itself. This included changes associated with ganglion cell death, reactive gliosis, and abortive regeneration. The second pattern of gene changes (23% of the variance) was primarily related to differences in gene expressions observed between the C57BL/6J and DBA/2J mouse strains. The remaining changes in gene expression represent interactions between the effects of optic nerve crush and the genetic background of the mouse. We extracted one genetic network from this dataset that appears to be related to tissue remodeling. One of the most intriguing sets of changes included members of the crystallin family of genes, which may represent a signature of pathways modulating the susceptibility of cells to death. Conclusion: Differential responses to optic nerve crush between two widely used strains of mice were used to define molecular networks associated with ganglion cell death and reactive gliosis. These results form the basis for our continuing interest in the modifiers of retinal injury.

Original languageEnglish (US)
Article number90
JournalBMC Neuroscience
Volume10
DOIs
StatePublished - Jul 30 2009

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Nerve Crush
Inbred DBA Mouse
Optic Nerve
Inbred C57BL Mouse
Cell Death
Ganglia
Gliosis
Retinal Ganglion Cells
Genes
Gene Expression
Crystallins
Inbred Strains Mice
Wounds and Injuries
Regeneration

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Cellular and Molecular Neuroscience

Cite this

Templeton, J. P., Nassr, M., Vazquez-Chona, F., Freeman-Anderson, N. E., Orr, W. E., Williams, R., & Geisert, E. E. (2009). Differential response of C57BL/6J mouse and DBA/2J mouse to optic nerve crush. BMC Neuroscience, 10, [90]. https://doi.org/10.1186/1471-2202-10-90

Differential response of C57BL/6J mouse and DBA/2J mouse to optic nerve crush. / Templeton, Justin P.; Nassr, Mohamed; Vazquez-Chona, Felix; Freeman-Anderson, Natalie E.; Orr, William E.; Williams, Robert; Geisert, Eldon E.

In: BMC Neuroscience, Vol. 10, 90, 30.07.2009.

Research output: Contribution to journalArticle

Templeton, JP, Nassr, M, Vazquez-Chona, F, Freeman-Anderson, NE, Orr, WE, Williams, R & Geisert, EE 2009, 'Differential response of C57BL/6J mouse and DBA/2J mouse to optic nerve crush', BMC Neuroscience, vol. 10, 90. https://doi.org/10.1186/1471-2202-10-90
Templeton JP, Nassr M, Vazquez-Chona F, Freeman-Anderson NE, Orr WE, Williams R et al. Differential response of C57BL/6J mouse and DBA/2J mouse to optic nerve crush. BMC Neuroscience. 2009 Jul 30;10. 90. https://doi.org/10.1186/1471-2202-10-90
Templeton, Justin P. ; Nassr, Mohamed ; Vazquez-Chona, Felix ; Freeman-Anderson, Natalie E. ; Orr, William E. ; Williams, Robert ; Geisert, Eldon E. / Differential response of C57BL/6J mouse and DBA/2J mouse to optic nerve crush. In: BMC Neuroscience. 2009 ; Vol. 10.
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