Differentially regulated malate synthase genes participate in carbon and nitrogen metabolism of S.cerevisiae

Andreas Hartig, Manuel M. Simon, Tillman Schuster, Jon R. Daugherty, Hyang Sook Yoo, Terrance Cooper

Research output: Contribution to journalArticle

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Abstract

We have isolated a second gene (MLS1), which in addition to DAL7, encodes malate synthase from S.cerevisiae. Expression of the two genes is specific for their physiological roles in carbon and nitrogen metabolism. Expression of MLS1, which participates in the utilization of non-fermentable carbon sources, is sensitive to carbon catabolite repression, but nearly insensitive to nitrogen catabolite repression. DAL7, which participates in catabolism of the nitrogenous compound allantoin, is insensitive to carbon catabolite repression, but highly sensitive to nitrogen catabolite repression. Results obtained with null mutations in these genes suggest that S.cerevisiae contains at least one and perhaps two additional malate synthase genes.

Original languageEnglish (US)
Pages (from-to)5677-5686
Number of pages10
JournalNucleic acids research
Volume20
Issue number21
DOIs
StatePublished - Nov 11 1992

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Malate Synthase
Catabolite Repression
Saccharomyces cerevisiae
Nitrogen
Carbon
Genes
Allantoin
Gene Expression
Mutation

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Differentially regulated malate synthase genes participate in carbon and nitrogen metabolism of S.cerevisiae. / Hartig, Andreas; Simon, Manuel M.; Schuster, Tillman; Daugherty, Jon R.; Yoo, Hyang Sook; Cooper, Terrance.

In: Nucleic acids research, Vol. 20, No. 21, 11.11.1992, p. 5677-5686.

Research output: Contribution to journalArticle

Hartig, Andreas ; Simon, Manuel M. ; Schuster, Tillman ; Daugherty, Jon R. ; Yoo, Hyang Sook ; Cooper, Terrance. / Differentially regulated malate synthase genes participate in carbon and nitrogen metabolism of S.cerevisiae. In: Nucleic acids research. 1992 ; Vol. 20, No. 21. pp. 5677-5686.
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