Differentiation of MC3T3-E1 Osteoblasts is associated with temporal changes in the expression of IGF-I and IGFBPs

K. M. Thrailkill, S. R. Siddhanti, J. L. Fowlkes, Leigh Quarles

Research output: Contribution to journalArticle

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Abstract

We examined the relationship between osteoblast maturation and temporal changes in the secretion of IGF-I and the IGF-binding proteins (IGFBPs) in the MC3T3-El model of osteoblast development. IGF-I was present at low levels in conditioned media in proliferating preosteoblasts (3.7 ± 1.7 ng/μg DNA and 3.9 ± 0.6 at culture (days 3 and 9) and increased progressively in postmitotic differentiating osteoblasts, reaching a maximal concentration of 13.1 ± 1.5 ng/μg DNA by day 25 of culture. We also observed an increase in IGF-I mRNA expression. Using Western ligand blot and immunoblot techniques, we found that IGFBP-2, -4, and -5 also displayed temporal differences in expression during MC3T3-El development. We observed a sustained increase in IGFBP-2, -4, and -5 mRNA expression between days 10-14, coincident with the onset of differentiation. IGFBP-2 and IGFBP-4 protein concentrations increased in parallel with IGFBP mRNA expression, but IGFBP-5 levels peaked between days 1-14 of culture, and declined thereafter in spite of persistent IGFBP-5 mRNA levels. These findings suggest complex transcriptional and post-transcriptional regulation of IGFBP metabolism during osteoblast development. Thus, IGF-I and IGFBP production are regulated during osteoblast development. In turn, time-dependent changes in IGF-I and modulation of IGF-I bioavailability by IGFBPs may regulate the osteoblastic developmental sequence.

Original languageEnglish (US)
Pages (from-to)307-313
Number of pages7
JournalBone
Volume17
Issue number3
DOIs
StatePublished - Jan 1 1995

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Insulin-Like Growth Factor Binding Proteins
Osteoblasts
Insulin-Like Growth Factor I
Insulin-Like Growth Factor Binding Protein 4
Insulin-Like Growth Factor Binding Protein 2
Insulin-Like Growth Factor Binding Protein 5
Messenger RNA
Western Blotting
DNA
Conditioned Culture Medium
Biological Availability
Ligands
Proteins

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Histology

Cite this

Differentiation of MC3T3-E1 Osteoblasts is associated with temporal changes in the expression of IGF-I and IGFBPs. / Thrailkill, K. M.; Siddhanti, S. R.; Fowlkes, J. L.; Quarles, Leigh.

In: Bone, Vol. 17, No. 3, 01.01.1995, p. 307-313.

Research output: Contribution to journalArticle

Thrailkill, K. M. ; Siddhanti, S. R. ; Fowlkes, J. L. ; Quarles, Leigh. / Differentiation of MC3T3-E1 Osteoblasts is associated with temporal changes in the expression of IGF-I and IGFBPs. In: Bone. 1995 ; Vol. 17, No. 3. pp. 307-313.
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