Dimers of the neuropeptide Y (NPY) Y2 receptor show asymmetry in agonist affinity and association with G proteins

M. S. Parker, R. Sah, A. Balasubramaniam, F. R. Sallee, T. Sweatman, Edwards Park, S. L. Parker

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In conditions precluding activation of G proteins, the binding of agonists to dimers of the neuropeptide Y (NPY) Y2 receptor shows two components of similar size, but differing in affinity. The dimers of all NPY receptors are solubilized as ∼ 180-kDa complexes containing one G protein α β γ trimer. These heteropentamers are stable to excess agonists, chelators, and alkylators. However, dispersion in the weak surfactant cholate releases ∼ 300-kDa complexes. These findings indicate that both protomers in the Y2 dimer are associated with G protein heterotrimers, but the extent of interaction depends on affinity for the agonist peptide. The G protein in contact with the first-liganded, higher-affinity protomer should have a stronger interaction with the receptor and a larger probability of activation.

Original languageEnglish (US)
Pages (from-to)437-451
Number of pages15
JournalJournal of Receptors and Signal Transduction
Volume28
Issue number5
DOIs
StatePublished - Sep 1 2008

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Neuropeptide Y Receptors
Neuropeptide Y
GTP-Binding Proteins
Dimers
Association reactions
Protein Subunits
Chemical activation
Cholates
Alkylating Agents
Chelating Agents
Protein Binding
Surface-Active Agents
Peptides
neuropeptide Y2 receptor

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Dimers of the neuropeptide Y (NPY) Y2 receptor show asymmetry in agonist affinity and association with G proteins. / Parker, M. S.; Sah, R.; Balasubramaniam, A.; Sallee, F. R.; Sweatman, T.; Park, Edwards; Parker, S. L.

In: Journal of Receptors and Signal Transduction, Vol. 28, No. 5, 01.09.2008, p. 437-451.

Research output: Contribution to journalArticle

Parker, M. S. ; Sah, R. ; Balasubramaniam, A. ; Sallee, F. R. ; Sweatman, T. ; Park, Edwards ; Parker, S. L. / Dimers of the neuropeptide Y (NPY) Y2 receptor show asymmetry in agonist affinity and association with G proteins. In: Journal of Receptors and Signal Transduction. 2008 ; Vol. 28, No. 5. pp. 437-451.
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