Direct effects of fatty acids and other charged lipids on ion channel activity in smooth muscle cells

S. Petrou, R. W. Ordway, M. T. Kirber, Alejandro Dopico, J. A. Hamilton, J. V. Walsh, J. J. Singer

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

A variety of fatty acids increase the activity of certain types of K+ channels. This effect is not dependent on the three enzymatic pathways that convert arachidonic acid to various bioactive oxygenated metabolites. One type of K+ channel in toad stomach smooth muscle cell membranes is activated by fatty acids and other single chain lipids which possess both a negatively charged head group and a sufficiently hydrophobic acyl chain. Neutral lipids have no effect on K+ channel activity, while positively charged lipids with a sufficiently hydrophobic acyl chain suppress channel activity. Acyl Coenzyme A's, which do not flip across the bilayer, act only from the cytosolic surface of the membrane, suggesting that the binding site for channel activation is also located there. This fatty acid-activated channel is also activated by membrane stretch. Moreover, this mechanical response is either mediated or modulated by fatty acids. Thus, fatty acids and other charged single chain lipids may comprise another class of first or second messenger molecules that target ion channels.

Original languageEnglish (US)
Pages (from-to)173-178
Number of pages6
JournalProstaglandins, Leukotrienes and Essential Fatty Acids
Volume52
Issue number2-3
DOIs
StatePublished - Jan 1 1995
Externally publishedYes

Fingerprint

Ion Channels
Smooth Muscle Myocytes
Muscle
Fatty Acids
Cells
Lipids
Membranes
Coenzymes
Second Messenger Systems
Cell membranes
Metabolites
Arachidonic Acid
Anura
Stomach
Chemical activation
Binding Sites
Cell Membrane
Molecules

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Cell Biology

Cite this

Direct effects of fatty acids and other charged lipids on ion channel activity in smooth muscle cells. / Petrou, S.; Ordway, R. W.; Kirber, M. T.; Dopico, Alejandro; Hamilton, J. A.; Walsh, J. V.; Singer, J. J.

In: Prostaglandins, Leukotrienes and Essential Fatty Acids, Vol. 52, No. 2-3, 01.01.1995, p. 173-178.

Research output: Contribution to journalArticle

Petrou, S. ; Ordway, R. W. ; Kirber, M. T. ; Dopico, Alejandro ; Hamilton, J. A. ; Walsh, J. V. ; Singer, J. J. / Direct effects of fatty acids and other charged lipids on ion channel activity in smooth muscle cells. In: Prostaglandins, Leukotrienes and Essential Fatty Acids. 1995 ; Vol. 52, No. 2-3. pp. 173-178.
@article{9de62d16928d4a67863ef2a5551bda1a,
title = "Direct effects of fatty acids and other charged lipids on ion channel activity in smooth muscle cells",
abstract = "A variety of fatty acids increase the activity of certain types of K+ channels. This effect is not dependent on the three enzymatic pathways that convert arachidonic acid to various bioactive oxygenated metabolites. One type of K+ channel in toad stomach smooth muscle cell membranes is activated by fatty acids and other single chain lipids which possess both a negatively charged head group and a sufficiently hydrophobic acyl chain. Neutral lipids have no effect on K+ channel activity, while positively charged lipids with a sufficiently hydrophobic acyl chain suppress channel activity. Acyl Coenzyme A's, which do not flip across the bilayer, act only from the cytosolic surface of the membrane, suggesting that the binding site for channel activation is also located there. This fatty acid-activated channel is also activated by membrane stretch. Moreover, this mechanical response is either mediated or modulated by fatty acids. Thus, fatty acids and other charged single chain lipids may comprise another class of first or second messenger molecules that target ion channels.",
author = "S. Petrou and Ordway, {R. W.} and Kirber, {M. T.} and Alejandro Dopico and Hamilton, {J. A.} and Walsh, {J. V.} and Singer, {J. J.}",
year = "1995",
month = "1",
day = "1",
doi = "10.1016/0952-3278(95)90018-7",
language = "English (US)",
volume = "52",
pages = "173--178",
journal = "Prostaglandins Leukotrienes and Essential Fatty Acids",
issn = "0952-3278",
publisher = "Churchill Livingstone",
number = "2-3",

}

TY - JOUR

T1 - Direct effects of fatty acids and other charged lipids on ion channel activity in smooth muscle cells

AU - Petrou, S.

AU - Ordway, R. W.

AU - Kirber, M. T.

AU - Dopico, Alejandro

AU - Hamilton, J. A.

AU - Walsh, J. V.

AU - Singer, J. J.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - A variety of fatty acids increase the activity of certain types of K+ channels. This effect is not dependent on the three enzymatic pathways that convert arachidonic acid to various bioactive oxygenated metabolites. One type of K+ channel in toad stomach smooth muscle cell membranes is activated by fatty acids and other single chain lipids which possess both a negatively charged head group and a sufficiently hydrophobic acyl chain. Neutral lipids have no effect on K+ channel activity, while positively charged lipids with a sufficiently hydrophobic acyl chain suppress channel activity. Acyl Coenzyme A's, which do not flip across the bilayer, act only from the cytosolic surface of the membrane, suggesting that the binding site for channel activation is also located there. This fatty acid-activated channel is also activated by membrane stretch. Moreover, this mechanical response is either mediated or modulated by fatty acids. Thus, fatty acids and other charged single chain lipids may comprise another class of first or second messenger molecules that target ion channels.

AB - A variety of fatty acids increase the activity of certain types of K+ channels. This effect is not dependent on the three enzymatic pathways that convert arachidonic acid to various bioactive oxygenated metabolites. One type of K+ channel in toad stomach smooth muscle cell membranes is activated by fatty acids and other single chain lipids which possess both a negatively charged head group and a sufficiently hydrophobic acyl chain. Neutral lipids have no effect on K+ channel activity, while positively charged lipids with a sufficiently hydrophobic acyl chain suppress channel activity. Acyl Coenzyme A's, which do not flip across the bilayer, act only from the cytosolic surface of the membrane, suggesting that the binding site for channel activation is also located there. This fatty acid-activated channel is also activated by membrane stretch. Moreover, this mechanical response is either mediated or modulated by fatty acids. Thus, fatty acids and other charged single chain lipids may comprise another class of first or second messenger molecules that target ion channels.

UR - http://www.scopus.com/inward/record.url?scp=0028815462&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028815462&partnerID=8YFLogxK

U2 - 10.1016/0952-3278(95)90018-7

DO - 10.1016/0952-3278(95)90018-7

M3 - Article

VL - 52

SP - 173

EP - 178

JO - Prostaglandins Leukotrienes and Essential Fatty Acids

JF - Prostaglandins Leukotrienes and Essential Fatty Acids

SN - 0952-3278

IS - 2-3

ER -