Disinfection of Streptococcus mutans biofilm by a non-thermal atmospheric plasma brush

Qing Hong, Xiaoqing Dong, Meng Chen, Yuanxi Xu, Hongmin Sun, Liang Hong, Yong Wang, Qingsong Yu

Research output: Contribution to journalArticle

Abstract

This study investigated the argon plasma treatment effect on disinfecting dental biofilm by using an atmospheric pressure plasma brush. Streptococcus mutans biofilms were developed for 3 days on the surfaces of hydroxyapatite (HA) discs, which were used to simulate human tooth enamel. After plasma treatment, cell viability in the S. mutans biofilms was characterized by using 3-(4,5-dimethylazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and confocal laser scanning microscopy (CLSM). Compared with the untreated control group, about 90% bacterial reduction in the biofilms was observed after 1 min plasma treatment. Scanning electron microscopy (SEM) examination indicated severe cell damages occurred on the top surface of the plasma treated biofilms. Confocal laser scanning microscopy (CLSM) showed that plasma treatment was effective as deep as 20 μm into the biofilms. When combined with antibiotic treatment using 0.2% chlorhexidine digluconate solution, the plasma treatment became more effective and over 96% bacterial reduction was observed with 1 min plasma treatment.

Original languageEnglish (US)
Article number07LG02
JournalJapanese Journal of Applied Physics
Volume55
Issue number7S2
DOIs
StatePublished - Jan 1 2016

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streptococcus
biofilms
Disinfection
brushes
Biofilms
Brushes
Plasmas
Microscopic examination
microscopy
Tooth enamel
Cells
scanning
enamels
Scanning
antibiotics
argon plasma
teeth
Lasers
viability
Antibiotics

All Science Journal Classification (ASJC) codes

  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Disinfection of Streptococcus mutans biofilm by a non-thermal atmospheric plasma brush. / Hong, Qing; Dong, Xiaoqing; Chen, Meng; Xu, Yuanxi; Sun, Hongmin; Hong, Liang; Wang, Yong; Yu, Qingsong.

In: Japanese Journal of Applied Physics, Vol. 55, No. 7S2, 07LG02, 01.01.2016.

Research output: Contribution to journalArticle

Hong, Qing ; Dong, Xiaoqing ; Chen, Meng ; Xu, Yuanxi ; Sun, Hongmin ; Hong, Liang ; Wang, Yong ; Yu, Qingsong. / Disinfection of Streptococcus mutans biofilm by a non-thermal atmospheric plasma brush. In: Japanese Journal of Applied Physics. 2016 ; Vol. 55, No. 7S2.
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