Distinct response of human B cell subpopulations in recognition of an innate immune signal, CpG DNA

Jaeho Jung, Ae-Kyung Yi, Xin Zhang, Jongseon Choe, Li Li, Yong Sung Choi

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Innate immunity has recently gained renewed interest in its ability to regulate adaptive immunity. Among the innate immune signals, CpG DNA has revealed its potential as a vaccine adjuvant. However, the cellular mechanism for the effect of CpG DNA on the humoral immune response is not well understood. Here, we investigated the effects of CpG DNA on human B cell differentiation using highly purified B cell subsets: naive, germinal center (GC), and memory B cells. In the in vitro culture system that mimics the primary or secondary immune response in vivo, CpG DNA markedly augmented the proliferation and generation of plasma cells from naive and memory B cells. CpG DNA dramatically increased plasma cell generation from GC B cells. However, CpG DNA did not have effect on memory B cell generation from GC B cells. These results suggest that CpG DNA potentiates the B cell adaptive immune response by enhancing terminal differentiation, but does not affect the generation of memory B cells.

Original languageEnglish (US)
Pages (from-to)2368-2373
Number of pages6
JournalJournal of Immunology
Volume169
Issue number5
DOIs
StatePublished - Sep 1 2002
Externally publishedYes

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B-Lymphocytes
DNA
Germinal Center
Adaptive Immunity
Plasma Cells
B-Lymphocyte Subsets
Humoral Immunity
Innate Immunity
Cell Differentiation
Vaccines

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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Distinct response of human B cell subpopulations in recognition of an innate immune signal, CpG DNA. / Jung, Jaeho; Yi, Ae-Kyung; Zhang, Xin; Choe, Jongseon; Li, Li; Choi, Yong Sung.

In: Journal of Immunology, Vol. 169, No. 5, 01.09.2002, p. 2368-2373.

Research output: Contribution to journalArticle

Jung, Jaeho ; Yi, Ae-Kyung ; Zhang, Xin ; Choe, Jongseon ; Li, Li ; Choi, Yong Sung. / Distinct response of human B cell subpopulations in recognition of an innate immune signal, CpG DNA. In: Journal of Immunology. 2002 ; Vol. 169, No. 5. pp. 2368-2373.
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