Distinctive serologic, chemical, and molecular properties of human λIV light chains

M. Eulitz, L. Y. Ch'ang, C. Zirkel, M. Schell, D. T. Weiss, Alan Solomon

Research output: Contribution to journalArticle

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Abstract

Through extensive serologic, chemical, and molecular studies involving monoclonal Ig proteins and B cell-related populations, we provide definitive evidence that the VλIV subgroup of human light (L) chains is separate and distinct from VλIII and all other known Vλ gene families. λIV and λIII L chains were differentiated immunochemically using well-characterized polyclonal and monoclonal anti-Vλ subgroup-specific Abs. Prototypic L chains, originally classified as λIV on the basis of distinctive framework region 1 residues, were distinguished serologically from λIIIa, λIIIb, and λIIIc proteins and also from λI, λII, λVI, and λVIII L chains. Furthermore, by using anti-λIV reagents, we identified eight additional monoclonal VλIV-related populations, including three IgM rheumatoid factor- producing cell lines. The percentage of homology among the λIV proteins ranged from 83 to 100, vs 53 to 72 when compared with λIII components. Moreover, λIV proteins shared particular subgroup-associated FR and complementarity-determining region residues. At the molecular level, the nucleotide sequences encoding two of the IgMλIV rheumatoid factors were identical to that found for the genomic counterpart, as well as to the previously reported IGLV3S1 and Humlv418 germ-line genes. Two other λIV cDNAs contained additional non-germline-encoded nucleotides at the V-J joint. The single or pauci-gene nature of the VλIV family was evidenced from Southern blotting and from the extensive sequence homology among λIV components. Our studies have provided further evidence for the prevalence of λIV L chains among Igλ autoantibodies, thus implying a functional significance for the VλIV subgroup.

Original languageEnglish (US)
Pages (from-to)3256-3265
Number of pages10
JournalJournal of Immunology
Volume154
Issue number7
StatePublished - Jan 1 1995

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Rheumatoid Factor
Light
Genes
Complementarity Determining Regions
Proteins
Sequence Homology
Southern Blotting
Germ Cells
Autoantibodies
Population
Immunoglobulin M
B-Lymphocytes
Nucleotides
Complementary DNA
Joints
Cell Line
IgA receptor

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Eulitz, M., Ch'ang, L. Y., Zirkel, C., Schell, M., Weiss, D. T., & Solomon, A. (1995). Distinctive serologic, chemical, and molecular properties of human λIV light chains. Journal of Immunology, 154(7), 3256-3265.

Distinctive serologic, chemical, and molecular properties of human λIV light chains. / Eulitz, M.; Ch'ang, L. Y.; Zirkel, C.; Schell, M.; Weiss, D. T.; Solomon, Alan.

In: Journal of Immunology, Vol. 154, No. 7, 01.01.1995, p. 3256-3265.

Research output: Contribution to journalArticle

Eulitz, M, Ch'ang, LY, Zirkel, C, Schell, M, Weiss, DT & Solomon, A 1995, 'Distinctive serologic, chemical, and molecular properties of human λIV light chains', Journal of Immunology, vol. 154, no. 7, pp. 3256-3265.
Eulitz M, Ch'ang LY, Zirkel C, Schell M, Weiss DT, Solomon A. Distinctive serologic, chemical, and molecular properties of human λIV light chains. Journal of Immunology. 1995 Jan 1;154(7):3256-3265.
Eulitz, M. ; Ch'ang, L. Y. ; Zirkel, C. ; Schell, M. ; Weiss, D. T. ; Solomon, Alan. / Distinctive serologic, chemical, and molecular properties of human λIV light chains. In: Journal of Immunology. 1995 ; Vol. 154, No. 7. pp. 3256-3265.
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