Distribution and clearance of cocaine in brain is influenced by genetics

Marc R. Azar, Nuket Acar, V. Gene Erwin, Guy F. Barbato, Andrew C. Morse, Carrie L. Heist, Byron Jones

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The purpose of this study was to examine the pharmacokinetics of cocaine in two inbred mouse strains, C57BL/6 (B6) and DBA/2 (D2). Male and female mice were administered 30 mg kg-1 cocaine IP and killed after 5, 15, 30, or 60 minutes postinjection. Brains were removed quickly and assayed for total brain cocaine concentration. Quantification of cocaine was conducted using gas chromatography and mass spectrometry. The results of this study revealed a strain difference in total brain cocaine kinetics. Specifically, we observed that at 5 min onward, B6 mice cleared cocaine from the brain with a t(1/2) estimated at 22.3 min, while distribution in D2 mice appeared to be incomplete until 15 min with a subsequent t(1/2) estimated at 11.2 min. These results show that despite faster clearance by D2 mice, the prolonged time to distribution in this strain may help explain why D2 mice show initial greater locomotor activation by cocaine, compared to B6s.

Original languageEnglish (US)
Pages (from-to)637-640
Number of pages4
JournalPharmacology Biochemistry and Behavior
Volume59
Issue number3
DOIs
StatePublished - Mar 1 1998

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Cocaine
Brain
Inbred DBA Mouse
Inbred Strains Mice
Pharmacokinetics
Genetics
Gas chromatography
Gas Chromatography-Mass Spectrometry
Mass spectrometry
Chemical activation
Kinetics

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Toxicology
  • Pharmacology
  • Clinical Biochemistry
  • Biological Psychiatry
  • Behavioral Neuroscience

Cite this

Distribution and clearance of cocaine in brain is influenced by genetics. / Azar, Marc R.; Acar, Nuket; Erwin, V. Gene; Barbato, Guy F.; Morse, Andrew C.; Heist, Carrie L.; Jones, Byron.

In: Pharmacology Biochemistry and Behavior, Vol. 59, No. 3, 01.03.1998, p. 637-640.

Research output: Contribution to journalArticle

Azar, Marc R. ; Acar, Nuket ; Erwin, V. Gene ; Barbato, Guy F. ; Morse, Andrew C. ; Heist, Carrie L. ; Jones, Byron. / Distribution and clearance of cocaine in brain is influenced by genetics. In: Pharmacology Biochemistry and Behavior. 1998 ; Vol. 59, No. 3. pp. 637-640.
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