Distribution of a tumor cell surface protein common to several murine lung carcinomas

Stephen Kennel, Patricia K. Lankford, Linda J. Foote, Frank S. Tsakeres, Laurel M. Adams, Robert L. Ullrich

Research output: Contribution to journalArticle

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Abstract

In previous work with line 1, a spontaneous BALB/c alveolar carcinoma, we identified a surface protein with a molecular weight of 180,000 (TSP-180) on tumor cells but not on normal mouse cells. This protein was not identical to known cell surface proteins or any C-type virus protein. In this report, we have continued to characterize TSP-180. It can be labeled metabolically with [3 H]leucine or [3 H]glucosamine, confirming that it is a glycoprotein synthesized by line 1 cells. In addition, a competition radioimmunoassay using radioiodinated TSP-180 from line 1 cells was used to measure the amount of TSP-180 in tissues of tumor-bearing animals as well as in other tissues and cells. It was found that during tumor growth, TSP-180 accumulates in the area of primary tumor to a plateau level. It is not detected in other organs except that small amounts are detected in serum when the primary tumor exceeds 1 cm in diameter. No evidence of cross-reactive material was found using BALB/c fibroblasts, mouse mammary tumor cells, BALB/c embryonic tissues, rat tracheal tumor cells, or human alveolar tumor cells. Complete competition was obtained using line 1 tissue culture cell or extracted tumor proteins. Carcinomas from two other mouse strains were also good competitors while adenomas of BALB/c lung competed weakly. Proteins from six other BALB/c lung carcinoma cell lines derived from radiation or chemically-induced tumors as well as tumors of spontaneous origin were also found to compete efficiently in the radioimmunoassay. The presence of TSP-180 on the surfaces of these lung carcinoma cell lines was confirmed using radioiodination catalyzed by lactoperoxidase. Partial protease digests of TSP-180 isolated from four of these lines showed identical molecular weight patterns indicating a highly conserved sequence homology. These results strongly suggest that TSP-180 represents a cell surface protein common to several tumors of the lung carcinoma class. The feasibility of using TSP-180 antigenic determinants for early detection of radiation or chemically-induced tumors, for monitoring progression of metastases, or as targets for directed drug delivery is discussed.

Original languageEnglish (US)
Pages (from-to)2153-2159
Number of pages7
JournalCancer Research
Volume40
Issue number7
StatePublished - Jul 1 1980
Externally publishedYes

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Integrin alpha6beta4
Membrane Proteins
Carcinoma
Lung
Neoplasms
Radioimmunoassay
Molecular Weight
Radiation
Bronchiolo-Alveolar Adenocarcinoma
Lactoperoxidase
Alveolar Epithelial Cells
Cell Line
Proteins
Conserved Sequence
Glucosamine
Sequence Homology
Protein C
Leucine
Adenoma
Epitopes

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Kennel, S., Lankford, P. K., Foote, L. J., Tsakeres, F. S., Adams, L. M., & Ullrich, R. L. (1980). Distribution of a tumor cell surface protein common to several murine lung carcinomas. Cancer Research, 40(7), 2153-2159.

Distribution of a tumor cell surface protein common to several murine lung carcinomas. / Kennel, Stephen; Lankford, Patricia K.; Foote, Linda J.; Tsakeres, Frank S.; Adams, Laurel M.; Ullrich, Robert L.

In: Cancer Research, Vol. 40, No. 7, 01.07.1980, p. 2153-2159.

Research output: Contribution to journalArticle

Kennel, S, Lankford, PK, Foote, LJ, Tsakeres, FS, Adams, LM & Ullrich, RL 1980, 'Distribution of a tumor cell surface protein common to several murine lung carcinomas', Cancer Research, vol. 40, no. 7, pp. 2153-2159.
Kennel S, Lankford PK, Foote LJ, Tsakeres FS, Adams LM, Ullrich RL. Distribution of a tumor cell surface protein common to several murine lung carcinomas. Cancer Research. 1980 Jul 1;40(7):2153-2159.
Kennel, Stephen ; Lankford, Patricia K. ; Foote, Linda J. ; Tsakeres, Frank S. ; Adams, Laurel M. ; Ullrich, Robert L. / Distribution of a tumor cell surface protein common to several murine lung carcinomas. In: Cancer Research. 1980 ; Vol. 40, No. 7. pp. 2153-2159.
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abstract = "In previous work with line 1, a spontaneous BALB/c alveolar carcinoma, we identified a surface protein with a molecular weight of 180,000 (TSP-180) on tumor cells but not on normal mouse cells. This protein was not identical to known cell surface proteins or any C-type virus protein. In this report, we have continued to characterize TSP-180. It can be labeled metabolically with [3 H]leucine or [3 H]glucosamine, confirming that it is a glycoprotein synthesized by line 1 cells. In addition, a competition radioimmunoassay using radioiodinated TSP-180 from line 1 cells was used to measure the amount of TSP-180 in tissues of tumor-bearing animals as well as in other tissues and cells. It was found that during tumor growth, TSP-180 accumulates in the area of primary tumor to a plateau level. It is not detected in other organs except that small amounts are detected in serum when the primary tumor exceeds 1 cm in diameter. No evidence of cross-reactive material was found using BALB/c fibroblasts, mouse mammary tumor cells, BALB/c embryonic tissues, rat tracheal tumor cells, or human alveolar tumor cells. Complete competition was obtained using line 1 tissue culture cell or extracted tumor proteins. Carcinomas from two other mouse strains were also good competitors while adenomas of BALB/c lung competed weakly. Proteins from six other BALB/c lung carcinoma cell lines derived from radiation or chemically-induced tumors as well as tumors of spontaneous origin were also found to compete efficiently in the radioimmunoassay. The presence of TSP-180 on the surfaces of these lung carcinoma cell lines was confirmed using radioiodination catalyzed by lactoperoxidase. Partial protease digests of TSP-180 isolated from four of these lines showed identical molecular weight patterns indicating a highly conserved sequence homology. These results strongly suggest that TSP-180 represents a cell surface protein common to several tumors of the lung carcinoma class. The feasibility of using TSP-180 antigenic determinants for early detection of radiation or chemically-induced tumors, for monitoring progression of metastases, or as targets for directed drug delivery is discussed.",
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