Distribution of angiotensin AT1 and AT2 receptor subtypes in the rat kidney

Noriyuki Miyata, Frank Park, Xiao Feng Li, Allen W. Cowley

Research output: Contribution to journalArticle

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Abstract

ANG II contributes importantly to the regulation of renal vascular resistance, glomerular filtration, and tubular epithelial transport, yet there remains a paucity of information regarding the localization of the ANG II type I and 2 (AT1 and AT2) receptors within the rat kidney particularly within the vasculature. The present study was designed to localize the transcriptional and translational site(s) of AT1 and AT2 receptor (AT1R and AT2R, respectively) expression within the rat kidney. Using immunohistochemistry, we detected the AT1R translational sites throughout the kidney, with the strongest labeling found in the vasculature of the renal cortex and the proximal tubules of the outer medulla. The AT2R protein expression was found throughout the rat kidney, although there was little to no expression found in the glomerulus and medullary thick ascending limbs of Henle (TAL). Gene-specific primers were then designed to distinguish between the receptor subtypes within microdissected renal tubular and vascular segments using RT-PCR. AT(1A)R, AT(1B)R, and AT2R mRNA were found within the renal vasculature (afferent arterioles, arcuate artery, and outer medullary descending vasa recta). The mRNA for both the AT1R isoforms was also detected in the glomeruli and the renal tubules (proximal tubules, TAL, and collecting ducts); however, no AT2R mRNA was detected within the glomerulus and was inconsistently found within the medullary TAL (MTAL). Taken together, these data show that mRNA for the AT1R subtypes was located in all of the renal tubular and vascular segments. Evidence for AT2R mRNA was also found in all but two of the vascular and tubular segments, the MTAL, and the glomeruli. These results are consistent with the whole tissue immunohistochemically localized receptors.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Physiology
Volume277
Issue number3 46-3
StatePublished - Sep 1 1999

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Angiotensin Type 2 Receptor
Angiotensin Type 1 Receptor
Kidney
Messenger RNA
Blood Vessels
Proximal Kidney Tubule
Extremities
Arterioles
Rectum
Vascular Resistance
Protein Isoforms
Arteries
Immunohistochemistry
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Physiology
  • Urology

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Distribution of angiotensin AT1 and AT2 receptor subtypes in the rat kidney. / Miyata, Noriyuki; Park, Frank; Li, Xiao Feng; Cowley, Allen W.

In: American Journal of Physiology - Renal Physiology, Vol. 277, No. 3 46-3, 01.09.1999.

Research output: Contribution to journalArticle

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