DNA binding site specificity of the Neurospora global nitrogen regulatory protein NIT2

Analysis with mutated binding sites

Tso Yu Chiang, Rajendra Rai, Terrance Cooper, George A. Marzluf

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

NIT2, a positive-acting regulatory protein in Neurospora crassa, activates the expression of a series of unlinked structural genes that encode nitrogen catabolic enzymes. NIT2 binding sites in the promoter regions of nit3, alc and lao have at least two GATA sequence elements. We have examined the binding affinity of the NIT2 protein for the yeast DAL5 wild-type upstream activation sequence UASNTR, which contains two GATA elements, and for a series of mutated binding sites, each differing from the wild-type site by a single base. Substitution for individual nucleotides within 5′ or 3′ sequences that flank the GATA elements had only modest effects upon NIT2 binding. In contrast, nearly all substitutions within the GATA elements almost completely eliminated NIT2 binding, demonstrating the importance of the GATA sequence for NIT2 binding. Four high-affinity binding sites for the NIT2 protein were found within a central region of the nit-2 gene itself.

Original languageEnglish (US)
Pages (from-to)512-516
Number of pages5
JournalMGG Molecular & General Genetics
Volume245
Issue number4
DOIs
StatePublished - Jul 1 1994

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Neurospora
Binding Sites
DNA
Laos
Pediculus
Neurospora crassa
Fungal Proteins
Genetic Promoter Regions
Genes
Proteins
Nitrogen
Nucleotides
Enzymes
Neurospora crassa NIT2 protein

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

DNA binding site specificity of the Neurospora global nitrogen regulatory protein NIT2 : Analysis with mutated binding sites. / Chiang, Tso Yu; Rai, Rajendra; Cooper, Terrance; Marzluf, George A.

In: MGG Molecular & General Genetics, Vol. 245, No. 4, 01.07.1994, p. 512-516.

Research output: Contribution to journalArticle

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