DNA microarrays

Their use and misuse

Xinmin Li, Weikuan Gu, Subburaman Mohan, David J. Baylink

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

DNA microarray represents one of the major advances in functional genomics. Ils ability to study expression of several thousands of genes or even all genes in the entire genome in a single experiment has changed the way in which we address basic biomedical questions. Numerous publications have shown its utility in drug discovery, disease diagnosis, novel gene identification, and understanding complex biological systems. However, there are substantive technical issues associated with the use of this technology that limit the interpretation of microarray data. In this review, we first give an overview of DNA microarray technology and then focus on uncertainty areas of microarray technology that include making microarrays, isolation of RNA and labeling, hybridization and scanning, and data analysis. The center theme of this review is to improve microarray reproducibility by addressing common technical problems. Finally, we briefly summarize microarray's applications in biomedical research.

Original languageEnglish (US)
Pages (from-to)13-22
Number of pages10
JournalMicrocirculation
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2002
Externally publishedYes

Fingerprint

Oligonucleotide Array Sequence Analysis
Technology
Genes
Drug Discovery
Genomics
Uncertainty
Publications
Biomedical Research
Genome
RNA

All Science Journal Classification (ASJC) codes

  • Physiology
  • Molecular Biology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

DNA microarrays : Their use and misuse. / Li, Xinmin; Gu, Weikuan; Mohan, Subburaman; Baylink, David J.

In: Microcirculation, Vol. 9, No. 1, 01.12.2002, p. 13-22.

Research output: Contribution to journalArticle

Li, X, Gu, W, Mohan, S & Baylink, DJ 2002, 'DNA microarrays: Their use and misuse', Microcirculation, vol. 9, no. 1, pp. 13-22. https://doi.org/10.1038/sj.mn.7800118
Li, Xinmin ; Gu, Weikuan ; Mohan, Subburaman ; Baylink, David J. / DNA microarrays : Their use and misuse. In: Microcirculation. 2002 ; Vol. 9, No. 1. pp. 13-22.
@article{fbb26641706c41ebbf6ef8c600bc7e35,
title = "DNA microarrays: Their use and misuse",
abstract = "DNA microarray represents one of the major advances in functional genomics. Ils ability to study expression of several thousands of genes or even all genes in the entire genome in a single experiment has changed the way in which we address basic biomedical questions. Numerous publications have shown its utility in drug discovery, disease diagnosis, novel gene identification, and understanding complex biological systems. However, there are substantive technical issues associated with the use of this technology that limit the interpretation of microarray data. In this review, we first give an overview of DNA microarray technology and then focus on uncertainty areas of microarray technology that include making microarrays, isolation of RNA and labeling, hybridization and scanning, and data analysis. The center theme of this review is to improve microarray reproducibility by addressing common technical problems. Finally, we briefly summarize microarray's applications in biomedical research.",
author = "Xinmin Li and Weikuan Gu and Subburaman Mohan and Baylink, {David J.}",
year = "2002",
month = "12",
day = "1",
doi = "10.1038/sj.mn.7800118",
language = "English (US)",
volume = "9",
pages = "13--22",
journal = "Microcirculation",
issn = "1073-9688",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - DNA microarrays

T2 - Their use and misuse

AU - Li, Xinmin

AU - Gu, Weikuan

AU - Mohan, Subburaman

AU - Baylink, David J.

PY - 2002/12/1

Y1 - 2002/12/1

N2 - DNA microarray represents one of the major advances in functional genomics. Ils ability to study expression of several thousands of genes or even all genes in the entire genome in a single experiment has changed the way in which we address basic biomedical questions. Numerous publications have shown its utility in drug discovery, disease diagnosis, novel gene identification, and understanding complex biological systems. However, there are substantive technical issues associated with the use of this technology that limit the interpretation of microarray data. In this review, we first give an overview of DNA microarray technology and then focus on uncertainty areas of microarray technology that include making microarrays, isolation of RNA and labeling, hybridization and scanning, and data analysis. The center theme of this review is to improve microarray reproducibility by addressing common technical problems. Finally, we briefly summarize microarray's applications in biomedical research.

AB - DNA microarray represents one of the major advances in functional genomics. Ils ability to study expression of several thousands of genes or even all genes in the entire genome in a single experiment has changed the way in which we address basic biomedical questions. Numerous publications have shown its utility in drug discovery, disease diagnosis, novel gene identification, and understanding complex biological systems. However, there are substantive technical issues associated with the use of this technology that limit the interpretation of microarray data. In this review, we first give an overview of DNA microarray technology and then focus on uncertainty areas of microarray technology that include making microarrays, isolation of RNA and labeling, hybridization and scanning, and data analysis. The center theme of this review is to improve microarray reproducibility by addressing common technical problems. Finally, we briefly summarize microarray's applications in biomedical research.

UR - http://www.scopus.com/inward/record.url?scp=0036369642&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036369642&partnerID=8YFLogxK

U2 - 10.1038/sj.mn.7800118

DO - 10.1038/sj.mn.7800118

M3 - Article

VL - 9

SP - 13

EP - 22

JO - Microcirculation

JF - Microcirculation

SN - 1073-9688

IS - 1

ER -