Do fish oil omega-3 fatty acids enhance antioxidant capacity and mitochondrial fatty acid oxidation in human atrial myocardium via PPARγ activation?

Ethan J. Anderson, Kathleen A. Thayne, Mitchel Harris, Saame Raza Shaikh, Timothy M. Darden, Daniel S. Lark, John Mark Williams, W. Randolph Chitwood, Alan P. Kypson, Evelio Rodriguez

    Research output: Contribution to journalReview article

    25 Citations (Scopus)

    Abstract

    Studies in experimental models suggest that n-3 polyunsaturated fatty acids (PUFAs) improve metabolic and anti-inflammatory/antioxidant capacity of the heart, although the mechanisms are unclear and translational evidence is lacking. In this study, patients ingested a moderately high dose of n-3 PUFAs (3.4g/day eicosapentaenoic (EPA) and doxosahexaenoic acid (DHA) ethyl-esters) for a period of 2-3 weeks before having elective cardiac surgery. Blood was obtained before treatment and at the time of surgery, and myocardial tissue from the right atrium was also dissected during surgery. Blood EPA levels increased and myocardial tissue EPA and DHA levels were significantly higher in n-3 PUFA-treated patients compared with untreated, standard-of-care control patients. Interestingly, n-3 PUFA patients had greater nuclear transactivation of peroxisome proliferator-activated receptor-γ (PPARγ), fatty acid metabolic gene expression, and enhanced mitochondrial respiration supported by palmitoyl-carnitine in the atrial myocardium, despite no difference in mitochondrial content. Myocardial tissue from n-3 PUFA patients also displayed greater expression and activity of key antioxidant/anti-inflammatory enzymes. These findings lead to our hypothesis that PPARγ activation is a mechanism by which fish oil n-3 PUFAs enhance mitochondrial fatty acid oxidation and antioxidant capacity in human atrial myocardium, and that this preoperative therapeutic regimen may be optimal for mitigating oxidative/inflammatory stress associated with cardiac surgery. Antioxid. Redox Signal. 21, 1156-1163.

    Original languageEnglish (US)
    Pages (from-to)1156-1163
    Number of pages8
    JournalAntioxidants and Redox Signaling
    Volume21
    Issue number8
    DOIs
    StatePublished - Sep 10 2014

    Fingerprint

    Peroxisome Proliferator-Activated Receptors
    Fish Oils
    Omega-3 Fatty Acids
    Myocardium
    Fatty Acids
    Antioxidants
    Chemical activation
    Oxidation
    Surgery
    Tissue
    Thoracic Surgery
    Blood
    Anti-Inflammatory Agents
    Oxidative stress
    Eicosapentaenoic Acid
    Carnitine
    Standard of Care
    Heart Atria
    Gene expression
    Transcriptional Activation

    All Science Journal Classification (ASJC) codes

    • Biochemistry
    • Cell Biology
    • Molecular Biology
    • Physiology
    • Clinical Biochemistry
    • Medicine(all)

    Cite this

    Do fish oil omega-3 fatty acids enhance antioxidant capacity and mitochondrial fatty acid oxidation in human atrial myocardium via PPARγ activation? / Anderson, Ethan J.; Thayne, Kathleen A.; Harris, Mitchel; Shaikh, Saame Raza; Darden, Timothy M.; Lark, Daniel S.; Williams, John Mark; Chitwood, W. Randolph; Kypson, Alan P.; Rodriguez, Evelio.

    In: Antioxidants and Redox Signaling, Vol. 21, No. 8, 10.09.2014, p. 1156-1163.

    Research output: Contribution to journalReview article

    Anderson, EJ, Thayne, KA, Harris, M, Shaikh, SR, Darden, TM, Lark, DS, Williams, JM, Chitwood, WR, Kypson, AP & Rodriguez, E 2014, 'Do fish oil omega-3 fatty acids enhance antioxidant capacity and mitochondrial fatty acid oxidation in human atrial myocardium via PPARγ activation?', Antioxidants and Redox Signaling, vol. 21, no. 8, pp. 1156-1163. https://doi.org/10.1089/ars.2014.5888
    Anderson, Ethan J. ; Thayne, Kathleen A. ; Harris, Mitchel ; Shaikh, Saame Raza ; Darden, Timothy M. ; Lark, Daniel S. ; Williams, John Mark ; Chitwood, W. Randolph ; Kypson, Alan P. ; Rodriguez, Evelio. / Do fish oil omega-3 fatty acids enhance antioxidant capacity and mitochondrial fatty acid oxidation in human atrial myocardium via PPARγ activation?. In: Antioxidants and Redox Signaling. 2014 ; Vol. 21, No. 8. pp. 1156-1163.
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    abstract = "Studies in experimental models suggest that n-3 polyunsaturated fatty acids (PUFAs) improve metabolic and anti-inflammatory/antioxidant capacity of the heart, although the mechanisms are unclear and translational evidence is lacking. In this study, patients ingested a moderately high dose of n-3 PUFAs (3.4g/day eicosapentaenoic (EPA) and doxosahexaenoic acid (DHA) ethyl-esters) for a period of 2-3 weeks before having elective cardiac surgery. Blood was obtained before treatment and at the time of surgery, and myocardial tissue from the right atrium was also dissected during surgery. Blood EPA levels increased and myocardial tissue EPA and DHA levels were significantly higher in n-3 PUFA-treated patients compared with untreated, standard-of-care control patients. Interestingly, n-3 PUFA patients had greater nuclear transactivation of peroxisome proliferator-activated receptor-γ (PPARγ), fatty acid metabolic gene expression, and enhanced mitochondrial respiration supported by palmitoyl-carnitine in the atrial myocardium, despite no difference in mitochondrial content. Myocardial tissue from n-3 PUFA patients also displayed greater expression and activity of key antioxidant/anti-inflammatory enzymes. These findings lead to our hypothesis that PPARγ activation is a mechanism by which fish oil n-3 PUFAs enhance mitochondrial fatty acid oxidation and antioxidant capacity in human atrial myocardium, and that this preoperative therapeutic regimen may be optimal for mitigating oxidative/inflammatory stress associated with cardiac surgery. Antioxid. Redox Signal. 21, 1156-1163.",
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    AU - Anderson, Ethan J.

    AU - Thayne, Kathleen A.

    AU - Harris, Mitchel

    AU - Shaikh, Saame Raza

    AU - Darden, Timothy M.

    AU - Lark, Daniel S.

    AU - Williams, John Mark

    AU - Chitwood, W. Randolph

    AU - Kypson, Alan P.

    AU - Rodriguez, Evelio

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    AB - Studies in experimental models suggest that n-3 polyunsaturated fatty acids (PUFAs) improve metabolic and anti-inflammatory/antioxidant capacity of the heart, although the mechanisms are unclear and translational evidence is lacking. In this study, patients ingested a moderately high dose of n-3 PUFAs (3.4g/day eicosapentaenoic (EPA) and doxosahexaenoic acid (DHA) ethyl-esters) for a period of 2-3 weeks before having elective cardiac surgery. Blood was obtained before treatment and at the time of surgery, and myocardial tissue from the right atrium was also dissected during surgery. Blood EPA levels increased and myocardial tissue EPA and DHA levels were significantly higher in n-3 PUFA-treated patients compared with untreated, standard-of-care control patients. Interestingly, n-3 PUFA patients had greater nuclear transactivation of peroxisome proliferator-activated receptor-γ (PPARγ), fatty acid metabolic gene expression, and enhanced mitochondrial respiration supported by palmitoyl-carnitine in the atrial myocardium, despite no difference in mitochondrial content. Myocardial tissue from n-3 PUFA patients also displayed greater expression and activity of key antioxidant/anti-inflammatory enzymes. These findings lead to our hypothesis that PPARγ activation is a mechanism by which fish oil n-3 PUFAs enhance mitochondrial fatty acid oxidation and antioxidant capacity in human atrial myocardium, and that this preoperative therapeutic regimen may be optimal for mitigating oxidative/inflammatory stress associated with cardiac surgery. Antioxid. Redox Signal. 21, 1156-1163.

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