Do liquid-based preparations of pulmonary bronchial brushing specimens perform differently from classically prepared cases for the diagnosis of malignancies?

Observations from the college of American pathologists interlaboratory comparison program in nongynecologic cytology

Z. Laura Tabatabai, Manon Auger, Daniel F.I. Kurtycz, Rodolfo Laucirica, Rhona J. Souers, Ritu Nayar, Walid E. Khalbuss, Ann T. Moriarty, Mostafa Fraig

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Context: Pulmonary bronchial brushing specimens can be processed by liquid-based or conventional methods. The ability to accurately diagnose a pulmonary malignancy with a liquid-based preparation (LBP) versus a conventional preparation may differ. Objective: To compare the performance of LBPs of malignant pulmonary bronchial brushing specimens with the performance of conventional preparations. Design: Participant responses from 553 malignant pulmonary bronchial brushing samples were evaluated for concordance with the general diagnosis. The performance of LBPs was compared with that of classic preparations. A nonlinear mixed model was used to analyze the performance by reference diagnosis, preparation type, program years, participant type, and the interaction terms between these 4 factors. Results: Concordance with the general category of malignant disease was observed in 95.2% of conventional Papanicolaou-stained, 90.9% of modified Giemsa-stained, and 96.9% of LBP (P<001) samples. The results were significantly different between individual reference diagnoses (P<.001). The performance of LBPs was consistently higher for most diagnoses and was significantly better for squamous cell carcinoma (P = .005), small cell carcinoma (P<001), and metastatic carcinoma not otherwise specified (P<.001). All participant types performed significantly better with LBPs of small cell carcinoma. Pathologists and cytotechnologists performed significantly better with LBPs of squamous cell carcinoma. A significantly higher concordance was observed between the general diagnosis and program years 2007-2011 versus 2001-2006 (P = .006). Conclusions: Liquid-based preparations performed better than conventional methods, with significantly higher performance in squamous cell, small cell, and metastatic carcinomas. Improved performance over time may reflect more frequent use of LBP methods and increased familiarity with interpreting the morphologic findings.

Original languageEnglish (US)
Pages (from-to)178-183
Number of pages6
JournalArchives of Pathology and Laboratory Medicine
Volume139
Issue number2
DOIs
StatePublished - Feb 1 2015

Fingerprint

Cell Biology
Lung
Small Cell Carcinoma
Neoplasms
Squamous Cell Carcinoma
Nonlinear Dynamics
Pathologists
Epithelial Cells
Carcinoma

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Medical Laboratory Technology

Cite this

Do liquid-based preparations of pulmonary bronchial brushing specimens perform differently from classically prepared cases for the diagnosis of malignancies? Observations from the college of American pathologists interlaboratory comparison program in nongynecologic cytology. / Tabatabai, Z. Laura; Auger, Manon; Kurtycz, Daniel F.I.; Laucirica, Rodolfo; Souers, Rhona J.; Nayar, Ritu; Khalbuss, Walid E.; Moriarty, Ann T.; Fraig, Mostafa.

In: Archives of Pathology and Laboratory Medicine, Vol. 139, No. 2, 01.02.2015, p. 178-183.

Research output: Contribution to journalArticle

@article{7d3e5511f33347d98a75f1e6168cd1e4,
title = "Do liquid-based preparations of pulmonary bronchial brushing specimens perform differently from classically prepared cases for the diagnosis of malignancies?: Observations from the college of American pathologists interlaboratory comparison program in nongynecologic cytology",
abstract = "Context: Pulmonary bronchial brushing specimens can be processed by liquid-based or conventional methods. The ability to accurately diagnose a pulmonary malignancy with a liquid-based preparation (LBP) versus a conventional preparation may differ. Objective: To compare the performance of LBPs of malignant pulmonary bronchial brushing specimens with the performance of conventional preparations. Design: Participant responses from 553 malignant pulmonary bronchial brushing samples were evaluated for concordance with the general diagnosis. The performance of LBPs was compared with that of classic preparations. A nonlinear mixed model was used to analyze the performance by reference diagnosis, preparation type, program years, participant type, and the interaction terms between these 4 factors. Results: Concordance with the general category of malignant disease was observed in 95.2{\%} of conventional Papanicolaou-stained, 90.9{\%} of modified Giemsa-stained, and 96.9{\%} of LBP (P<001) samples. The results were significantly different between individual reference diagnoses (P<.001). The performance of LBPs was consistently higher for most diagnoses and was significantly better for squamous cell carcinoma (P = .005), small cell carcinoma (P<001), and metastatic carcinoma not otherwise specified (P<.001). All participant types performed significantly better with LBPs of small cell carcinoma. Pathologists and cytotechnologists performed significantly better with LBPs of squamous cell carcinoma. A significantly higher concordance was observed between the general diagnosis and program years 2007-2011 versus 2001-2006 (P = .006). Conclusions: Liquid-based preparations performed better than conventional methods, with significantly higher performance in squamous cell, small cell, and metastatic carcinomas. Improved performance over time may reflect more frequent use of LBP methods and increased familiarity with interpreting the morphologic findings.",
author = "Tabatabai, {Z. Laura} and Manon Auger and Kurtycz, {Daniel F.I.} and Rodolfo Laucirica and Souers, {Rhona J.} and Ritu Nayar and Khalbuss, {Walid E.} and Moriarty, {Ann T.} and Mostafa Fraig",
year = "2015",
month = "2",
day = "1",
doi = "10.5858/arpa.2013-0282-CP",
language = "English (US)",
volume = "139",
pages = "178--183",
journal = "Archives of Pathology and Laboratory Medicine",
issn = "0003-9985",
publisher = "College of American Pathologists",
number = "2",

}

TY - JOUR

T1 - Do liquid-based preparations of pulmonary bronchial brushing specimens perform differently from classically prepared cases for the diagnosis of malignancies?

T2 - Observations from the college of American pathologists interlaboratory comparison program in nongynecologic cytology

AU - Tabatabai, Z. Laura

AU - Auger, Manon

AU - Kurtycz, Daniel F.I.

AU - Laucirica, Rodolfo

AU - Souers, Rhona J.

AU - Nayar, Ritu

AU - Khalbuss, Walid E.

AU - Moriarty, Ann T.

AU - Fraig, Mostafa

PY - 2015/2/1

Y1 - 2015/2/1

N2 - Context: Pulmonary bronchial brushing specimens can be processed by liquid-based or conventional methods. The ability to accurately diagnose a pulmonary malignancy with a liquid-based preparation (LBP) versus a conventional preparation may differ. Objective: To compare the performance of LBPs of malignant pulmonary bronchial brushing specimens with the performance of conventional preparations. Design: Participant responses from 553 malignant pulmonary bronchial brushing samples were evaluated for concordance with the general diagnosis. The performance of LBPs was compared with that of classic preparations. A nonlinear mixed model was used to analyze the performance by reference diagnosis, preparation type, program years, participant type, and the interaction terms between these 4 factors. Results: Concordance with the general category of malignant disease was observed in 95.2% of conventional Papanicolaou-stained, 90.9% of modified Giemsa-stained, and 96.9% of LBP (P<001) samples. The results were significantly different between individual reference diagnoses (P<.001). The performance of LBPs was consistently higher for most diagnoses and was significantly better for squamous cell carcinoma (P = .005), small cell carcinoma (P<001), and metastatic carcinoma not otherwise specified (P<.001). All participant types performed significantly better with LBPs of small cell carcinoma. Pathologists and cytotechnologists performed significantly better with LBPs of squamous cell carcinoma. A significantly higher concordance was observed between the general diagnosis and program years 2007-2011 versus 2001-2006 (P = .006). Conclusions: Liquid-based preparations performed better than conventional methods, with significantly higher performance in squamous cell, small cell, and metastatic carcinomas. Improved performance over time may reflect more frequent use of LBP methods and increased familiarity with interpreting the morphologic findings.

AB - Context: Pulmonary bronchial brushing specimens can be processed by liquid-based or conventional methods. The ability to accurately diagnose a pulmonary malignancy with a liquid-based preparation (LBP) versus a conventional preparation may differ. Objective: To compare the performance of LBPs of malignant pulmonary bronchial brushing specimens with the performance of conventional preparations. Design: Participant responses from 553 malignant pulmonary bronchial brushing samples were evaluated for concordance with the general diagnosis. The performance of LBPs was compared with that of classic preparations. A nonlinear mixed model was used to analyze the performance by reference diagnosis, preparation type, program years, participant type, and the interaction terms between these 4 factors. Results: Concordance with the general category of malignant disease was observed in 95.2% of conventional Papanicolaou-stained, 90.9% of modified Giemsa-stained, and 96.9% of LBP (P<001) samples. The results were significantly different between individual reference diagnoses (P<.001). The performance of LBPs was consistently higher for most diagnoses and was significantly better for squamous cell carcinoma (P = .005), small cell carcinoma (P<001), and metastatic carcinoma not otherwise specified (P<.001). All participant types performed significantly better with LBPs of small cell carcinoma. Pathologists and cytotechnologists performed significantly better with LBPs of squamous cell carcinoma. A significantly higher concordance was observed between the general diagnosis and program years 2007-2011 versus 2001-2006 (P = .006). Conclusions: Liquid-based preparations performed better than conventional methods, with significantly higher performance in squamous cell, small cell, and metastatic carcinomas. Improved performance over time may reflect more frequent use of LBP methods and increased familiarity with interpreting the morphologic findings.

UR - http://www.scopus.com/inward/record.url?scp=84921502871&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84921502871&partnerID=8YFLogxK

U2 - 10.5858/arpa.2013-0282-CP

DO - 10.5858/arpa.2013-0282-CP

M3 - Article

VL - 139

SP - 178

EP - 183

JO - Archives of Pathology and Laboratory Medicine

JF - Archives of Pathology and Laboratory Medicine

SN - 0003-9985

IS - 2

ER -