Dopamine Regulates Aversive Contextual Learning and Associated In Vivo Synaptic Plasticity in the Hippocampus

John I. Broussard, Kechun Yang, Amber T. Levine, Theodoros Tsetsenis, Daniel Jenson, Fei Cao, Isabella Garcia, Benjamin R. Arenkiel, Fuming Zhou, Mariella De Biasi, John A. Dani

Research output: Contribution to journalArticle

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Abstract

Dopamine release during reward-driven behaviors influences synaptic plasticity. However, dopamine innervation and release in the hippocampus and its role during aversive behaviors are controversial. Here, we show that in vivo hippocampal synaptic plasticity in the CA3-CA1 circuit underlies contextual learning during inhibitory avoidance (IA) training. Immunohistochemistry and molecular techniques verified sparse dopaminergic innervation of the hippocampus from the midbrain. The long-term synaptic potentiation (LTP) underlying the learning of IA was assessed with a D1-like dopamine receptor agonist or antagonist in ex vivo hippocampal slices and in vivo in freely moving mice. Inhibition of D1-like dopamine receptors impaired memory of the IA task and prevented the training-induced enhancement of both ex vivo and in vivo LTP induction. The results indicate that dopamine-receptor signaling during an aversive contextual task regulates aversive memory retention and regulates associated synaptic mechanisms in the hippocampus that likely underlie learning.

Original languageEnglish (US)
Pages (from-to)1930-1939
Number of pages10
JournalCell Reports
Volume14
Issue number8
DOIs
StatePublished - Mar 1 2016

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Neuronal Plasticity
Dopamine Receptors
Plasticity
Hippocampus
Dopamine
Long-Term Potentiation
Learning
Data storage equipment
Dopamine Antagonists
Dopamine Agonists
Avoidance Learning
Dopamine D1 Receptors
Mesencephalon
Reward
Networks (circuits)
Immunohistochemistry

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Broussard, J. I., Yang, K., Levine, A. T., Tsetsenis, T., Jenson, D., Cao, F., ... Dani, J. A. (2016). Dopamine Regulates Aversive Contextual Learning and Associated In Vivo Synaptic Plasticity in the Hippocampus. Cell Reports, 14(8), 1930-1939. https://doi.org/10.1016/j.celrep.2016.01.070

Dopamine Regulates Aversive Contextual Learning and Associated In Vivo Synaptic Plasticity in the Hippocampus. / Broussard, John I.; Yang, Kechun; Levine, Amber T.; Tsetsenis, Theodoros; Jenson, Daniel; Cao, Fei; Garcia, Isabella; Arenkiel, Benjamin R.; Zhou, Fuming; De Biasi, Mariella; Dani, John A.

In: Cell Reports, Vol. 14, No. 8, 01.03.2016, p. 1930-1939.

Research output: Contribution to journalArticle

Broussard, JI, Yang, K, Levine, AT, Tsetsenis, T, Jenson, D, Cao, F, Garcia, I, Arenkiel, BR, Zhou, F, De Biasi, M & Dani, JA 2016, 'Dopamine Regulates Aversive Contextual Learning and Associated In Vivo Synaptic Plasticity in the Hippocampus', Cell Reports, vol. 14, no. 8, pp. 1930-1939. https://doi.org/10.1016/j.celrep.2016.01.070
Broussard, John I. ; Yang, Kechun ; Levine, Amber T. ; Tsetsenis, Theodoros ; Jenson, Daniel ; Cao, Fei ; Garcia, Isabella ; Arenkiel, Benjamin R. ; Zhou, Fuming ; De Biasi, Mariella ; Dani, John A. / Dopamine Regulates Aversive Contextual Learning and Associated In Vivo Synaptic Plasticity in the Hippocampus. In: Cell Reports. 2016 ; Vol. 14, No. 8. pp. 1930-1939.
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