Dual action of intracellularly released calcium on the quantal mediator secretion

O. P. Balezina, Anna Bukiya, V. I. Lapteva

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The mice diaphragm muscle and microelectrode technique were used to check the influence of ryanodine (0.5 mcM) on spontaneous and evoked mediator release under conditions of potassium depolarization (8-16 mM [K+]ex or rhythmic (4-100 Hz) stimulation of motor nerve terminals. Weak tonic calcium loading (by muscle exposition to 8 mM [K+]ex) caused a two-fold frequency increase if miniature and plate potentials (MEPPs), which was returned to the basal level by subsequent application of ryanodine. This inhibitory effect of ryanodine was blocked by apamin (500 nM) a blocker of K+(Ca)-channels. A greater calcium load of terminals (in solution with 16 mM [K+]ex) caused a 15-fold increase of MEPPs frequency. Subsequent ryanodine application caused an additional 2-3-fold increase of MEPPs frequency. During rhythmic activity of motor synapses, ryanodine was able to decrease the amplitude of EPP by 60% at plateau phase at short low frequency (4 Hz) of discharges and to increase the amplitude of EPP by 60-150% at high frequency (70-100 Hz) of discharges. It is concluded that rynodine induced calcium release from intraterminal Ca2+-stores can influence dual: excitatory or inhibitory, action on spontaneous and evoked mediator release, due to different intraterminal calcium loads and regimen of synaptic activity.

Original languageEnglish (US)
Pages (from-to)61-70
Number of pages10
JournalRossiiskii fiziologicheskii zhurnal imeni I.M. Sechenova / Rossiiskaia akademiia nauk
Volume91
Issue number1
StatePublished - Jan 1 2005

Fingerprint

Ryanodine
Calcium
Apamin
Muscles
Microelectrodes
Diaphragm
Synapses
Potassium
Motor Activity

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Dual action of intracellularly released calcium on the quantal mediator secretion. / Balezina, O. P.; Bukiya, Anna; Lapteva, V. I.

In: Rossiiskii fiziologicheskii zhurnal imeni I.M. Sechenova / Rossiiskaia akademiia nauk, Vol. 91, No. 1, 01.01.2005, p. 61-70.

Research output: Contribution to journalArticle

@article{87e9a9584d254d019bd4edd82a684f14,
title = "Dual action of intracellularly released calcium on the quantal mediator secretion",
abstract = "The mice diaphragm muscle and microelectrode technique were used to check the influence of ryanodine (0.5 mcM) on spontaneous and evoked mediator release under conditions of potassium depolarization (8-16 mM [K+]ex or rhythmic (4-100 Hz) stimulation of motor nerve terminals. Weak tonic calcium loading (by muscle exposition to 8 mM [K+]ex) caused a two-fold frequency increase if miniature and plate potentials (MEPPs), which was returned to the basal level by subsequent application of ryanodine. This inhibitory effect of ryanodine was blocked by apamin (500 nM) a blocker of K+(Ca)-channels. A greater calcium load of terminals (in solution with 16 mM [K+]ex) caused a 15-fold increase of MEPPs frequency. Subsequent ryanodine application caused an additional 2-3-fold increase of MEPPs frequency. During rhythmic activity of motor synapses, ryanodine was able to decrease the amplitude of EPP by 60{\%} at plateau phase at short low frequency (4 Hz) of discharges and to increase the amplitude of EPP by 60-150{\%} at high frequency (70-100 Hz) of discharges. It is concluded that rynodine induced calcium release from intraterminal Ca2+-stores can influence dual: excitatory or inhibitory, action on spontaneous and evoked mediator release, due to different intraterminal calcium loads and regimen of synaptic activity.",
author = "Balezina, {O. P.} and Anna Bukiya and Lapteva, {V. I.}",
year = "2005",
month = "1",
day = "1",
language = "English (US)",
volume = "91",
pages = "61--70",
journal = "Fiziologicheskii Zhurnal Imeni I.M. Sechenova",
issn = "0869-8139",
publisher = "Nauka Publishers",
number = "1",

}

TY - JOUR

T1 - Dual action of intracellularly released calcium on the quantal mediator secretion

AU - Balezina, O. P.

AU - Bukiya, Anna

AU - Lapteva, V. I.

PY - 2005/1/1

Y1 - 2005/1/1

N2 - The mice diaphragm muscle and microelectrode technique were used to check the influence of ryanodine (0.5 mcM) on spontaneous and evoked mediator release under conditions of potassium depolarization (8-16 mM [K+]ex or rhythmic (4-100 Hz) stimulation of motor nerve terminals. Weak tonic calcium loading (by muscle exposition to 8 mM [K+]ex) caused a two-fold frequency increase if miniature and plate potentials (MEPPs), which was returned to the basal level by subsequent application of ryanodine. This inhibitory effect of ryanodine was blocked by apamin (500 nM) a blocker of K+(Ca)-channels. A greater calcium load of terminals (in solution with 16 mM [K+]ex) caused a 15-fold increase of MEPPs frequency. Subsequent ryanodine application caused an additional 2-3-fold increase of MEPPs frequency. During rhythmic activity of motor synapses, ryanodine was able to decrease the amplitude of EPP by 60% at plateau phase at short low frequency (4 Hz) of discharges and to increase the amplitude of EPP by 60-150% at high frequency (70-100 Hz) of discharges. It is concluded that rynodine induced calcium release from intraterminal Ca2+-stores can influence dual: excitatory or inhibitory, action on spontaneous and evoked mediator release, due to different intraterminal calcium loads and regimen of synaptic activity.

AB - The mice diaphragm muscle and microelectrode technique were used to check the influence of ryanodine (0.5 mcM) on spontaneous and evoked mediator release under conditions of potassium depolarization (8-16 mM [K+]ex or rhythmic (4-100 Hz) stimulation of motor nerve terminals. Weak tonic calcium loading (by muscle exposition to 8 mM [K+]ex) caused a two-fold frequency increase if miniature and plate potentials (MEPPs), which was returned to the basal level by subsequent application of ryanodine. This inhibitory effect of ryanodine was blocked by apamin (500 nM) a blocker of K+(Ca)-channels. A greater calcium load of terminals (in solution with 16 mM [K+]ex) caused a 15-fold increase of MEPPs frequency. Subsequent ryanodine application caused an additional 2-3-fold increase of MEPPs frequency. During rhythmic activity of motor synapses, ryanodine was able to decrease the amplitude of EPP by 60% at plateau phase at short low frequency (4 Hz) of discharges and to increase the amplitude of EPP by 60-150% at high frequency (70-100 Hz) of discharges. It is concluded that rynodine induced calcium release from intraterminal Ca2+-stores can influence dual: excitatory or inhibitory, action on spontaneous and evoked mediator release, due to different intraterminal calcium loads and regimen of synaptic activity.

UR - http://www.scopus.com/inward/record.url?scp=20344396615&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=20344396615&partnerID=8YFLogxK

M3 - Article

C2 - 15773581

AN - SCOPUS:20344396615

VL - 91

SP - 61

EP - 70

JO - Fiziologicheskii Zhurnal Imeni I.M. Sechenova

JF - Fiziologicheskii Zhurnal Imeni I.M. Sechenova

SN - 0869-8139

IS - 1

ER -