Dynamics of ankyrin-containing complexes in chicken embryonic erythroid cells

Role of phosphorylation

S. Ghosh, John Cox

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Chicken erythroid ankyrin undergoes a fairly rapid cycle of cytoskeletal association, dissociation, and turnover. In addition, the cytoskeletal association of ankyrin is regulated by phosphorylation. Treatment of erythroid cells with serine and threonine phosphatase inhibitors stimulated the hyperphosphorylation of the 225- and 205-kDa ankyrin isoforms, and dissociated the bulk of these isoforms from cytoskeletal spectrin. In vitro binding studies have shown that this dissociation of ankyrin from spectrin in vivo can be attributed to a reduced ability of hyperphosphorylated ankyrin to bind spectrin. Interestingly, a significant fraction of detergent insoluble ankyrin accumulates in a spectrin-independent pool. At least some of this spectrin-independent pool of ankyrin is complexed with the AE1 anion exchanger, and the solubility properties of this pool are also regulated by phosphorylation. Treatment of cells with serine and threonine phosphatase inhibitors had no effect on ankyrin/AE1 complex formation. However, these inhibitors were sufficient to shift ankyrin/AE1 complexes from the detergent insoluble to the soluble pool. These analyses, which are the first to document the in vivo consequences of ankyrin phosphorylation, indicate that erythroid ankyrin-containing complexes can undergo dynamic rearrangements in response to changes in phosphorylation.

Original languageEnglish (US)
Pages (from-to)3864-3874
Number of pages11
JournalMolecular Biology of the Cell
Volume12
Issue number12
DOIs
StatePublished - Jan 1 2001

Fingerprint

Ankyrins
Erythroid Cells
Chickens
Phosphorylation
Spectrin
Phosphoprotein Phosphatases
Detergents
Protein Isoforms
Erythrocyte Anion Exchange Protein 1
Solubility

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Dynamics of ankyrin-containing complexes in chicken embryonic erythroid cells : Role of phosphorylation. / Ghosh, S.; Cox, John.

In: Molecular Biology of the Cell, Vol. 12, No. 12, 01.01.2001, p. 3864-3874.

Research output: Contribution to journalArticle

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