Dynamics of nuclear localization sites for COX-2 in vascular endothelial cells

Elena Parfenova, Vladimir N. Parfenov, Boris V. Shlopov, Vladimir Levine, Sheryl Falkos, Massroor Pourcyrous, Charles Leffler

Research output: Contribution to journalArticle

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Abstract

We investigated the relationships among expression, activity, and spatial organization of cyclooxygenase (COX-1 and COX-2) in endothelial cells from porcine and human cerebral microvessels and from human umbilical vein. In quiescent cells, COX-1 was detected in the perinuclear zone and the cytoplasm, while COX-2 was mainly a nuclear resident possibly connected with the nuclear matrix. COX-2 immunogold labeling was situated in the nuclear envelope, at the nuclear pores, and in connection with the perichromatin regions of the nucleus, considered to be the sites of active transcription. In human endothelial cells transcriptionally activated by interleukin (IL)-1β, the nucleus remained a major COX-2 localization site during the first 12 h of stimulation, when COX-2 expression was maximally induced. The continuous rise in prostanoid synthesis at 17-23 h of stimulation was associated with COX-2 relocation from the nucleus to the nuclear envelope and the cytoplasm. IL-1β did not affect COX-1 expression, activity, and localization. COX-2 nuclear localization sites and trafficking between the nucleus and the cytoplasm in endothelial cells may indicate a novel function of COX-2 in regulating gene expression.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume281
Issue number1 50-1
StatePublished - Oct 16 2001

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Cytoplasm
Endothelial Cells
Nuclear Envelope
Interleukin-1
Nuclear Matrix
Nuclear Pore
Cyclooxygenase 1
Umbilical Veins
Microvessels
Prostaglandins
Catalytic Domain
Swine
Gene Expression

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cell Biology

Cite this

Dynamics of nuclear localization sites for COX-2 in vascular endothelial cells. / Parfenova, Elena; Parfenov, Vladimir N.; Shlopov, Boris V.; Levine, Vladimir; Falkos, Sheryl; Pourcyrous, Massroor; Leffler, Charles.

In: American Journal of Physiology - Cell Physiology, Vol. 281, No. 1 50-1, 16.10.2001.

Research output: Contribution to journalArticle

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AU - Parfenova, Elena

AU - Parfenov, Vladimir N.

AU - Shlopov, Boris V.

AU - Levine, Vladimir

AU - Falkos, Sheryl

AU - Pourcyrous, Massroor

AU - Leffler, Charles

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N2 - We investigated the relationships among expression, activity, and spatial organization of cyclooxygenase (COX-1 and COX-2) in endothelial cells from porcine and human cerebral microvessels and from human umbilical vein. In quiescent cells, COX-1 was detected in the perinuclear zone and the cytoplasm, while COX-2 was mainly a nuclear resident possibly connected with the nuclear matrix. COX-2 immunogold labeling was situated in the nuclear envelope, at the nuclear pores, and in connection with the perichromatin regions of the nucleus, considered to be the sites of active transcription. In human endothelial cells transcriptionally activated by interleukin (IL)-1β, the nucleus remained a major COX-2 localization site during the first 12 h of stimulation, when COX-2 expression was maximally induced. The continuous rise in prostanoid synthesis at 17-23 h of stimulation was associated with COX-2 relocation from the nucleus to the nuclear envelope and the cytoplasm. IL-1β did not affect COX-1 expression, activity, and localization. COX-2 nuclear localization sites and trafficking between the nucleus and the cytoplasm in endothelial cells may indicate a novel function of COX-2 in regulating gene expression.

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