Dystrophin-glycoprotein complex and Ras and Rho GTPase signaling are altered in muscle atrophy

Priya Sethu Chockalingam, Rushina Cholera, Shilpa A. Oak, Yi Zheng, Harry W. Jarrett, Donald B. Thomason

Research output: Contribution to journalArticle

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Abstract

The dystrophinglycoprotein complex (DGC) is a sarcolemmal complex whose defects cause muscular dystrophies. The normal function of this complex is not clear. We have proposed that this is a signal transduction complex, signaling normal interactions with matrix laminin, and that the response is normal growth and homeostasis. If so, the complex and its signaling should be altered in other physiological states such as atrophy. The amount of some of the DGC proteins, including dystrophin, β-dystroglycan, and α-sarcoglycan, is reduced significantly in rat skeletal muscle atrophy induced by tenotomy. Furthermore, H-Ras, RhoA, and Cdc42 decrease in expression levels and activities in muscle atrophy. When the small GTPases were assayed after laminin or β-dystroglycan depletion, H-Ras, Rac1, and Cdc42 activities were reduced, suggesting a physical linkage between the DGC and the GTPases. Dominant-negative Cdc42, introduced with a retroviral vector, resulted in fibers that appeared atrophic. These data support a putative role for the DGC in transduction of mechanical signals in muscle.

Original languageEnglish (US)
Pages (from-to)C500-C511
JournalAmerican Journal of Physiology - Cell Physiology
Volume283
Issue number2 52-2
StatePublished - Jul 30 2002

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Dystroglycans
ras Proteins
rho GTP-Binding Proteins
Dystrophin
Muscular Atrophy
Laminin
Muscle
Glycoproteins
Cellular Mechanotransduction
Sarcoglycans
Tenotomy
Monomeric GTP-Binding Proteins
Muscular Dystrophies
GTP Phosphohydrolases
Atrophy
Signal Transduction
Signal transduction
Skeletal Muscle
Homeostasis
Muscles

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cell Biology

Cite this

Chockalingam, P. S., Cholera, R., Oak, S. A., Zheng, Y., Jarrett, H. W., & Thomason, D. B. (2002). Dystrophin-glycoprotein complex and Ras and Rho GTPase signaling are altered in muscle atrophy. American Journal of Physiology - Cell Physiology, 283(2 52-2), C500-C511.

Dystrophin-glycoprotein complex and Ras and Rho GTPase signaling are altered in muscle atrophy. / Chockalingam, Priya Sethu; Cholera, Rushina; Oak, Shilpa A.; Zheng, Yi; Jarrett, Harry W.; Thomason, Donald B.

In: American Journal of Physiology - Cell Physiology, Vol. 283, No. 2 52-2, 30.07.2002, p. C500-C511.

Research output: Contribution to journalArticle

Chockalingam, PS, Cholera, R, Oak, SA, Zheng, Y, Jarrett, HW & Thomason, DB 2002, 'Dystrophin-glycoprotein complex and Ras and Rho GTPase signaling are altered in muscle atrophy', American Journal of Physiology - Cell Physiology, vol. 283, no. 2 52-2, pp. C500-C511.
Chockalingam, Priya Sethu ; Cholera, Rushina ; Oak, Shilpa A. ; Zheng, Yi ; Jarrett, Harry W. ; Thomason, Donald B. / Dystrophin-glycoprotein complex and Ras and Rho GTPase signaling are altered in muscle atrophy. In: American Journal of Physiology - Cell Physiology. 2002 ; Vol. 283, No. 2 52-2. pp. C500-C511.
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