Early gene expression events in ferrets in response to SARS coronavirus infection versus direct interferon-alpha2b stimulation

Ali Danesh, Cheryl M. Cameron, Alberto J. León, Longsi Ran, Luoling Xu, Yuan Fang, Alyson A. Kelvin, Thomas Rowe, Honglin Chen, Yi Guan, Colleen Jonsson, Mark J. Cameron, David J. Kelvin

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Type I interferons (IFNs) are essential to the clearance of viral diseases, however, a clear distinction between genes upregulated by direct virus-cell interactions and genes upregulated by secondary IFN production has not been made. Here, we investigated differential gene regulation in ferrets upon subcutaneous administration of IFN-α2b and during SARS-CoV infection. In vivo experiments revealed that IFN-α2b causes STAT1 phosphorylation and upregulation of abundant IFN response genes (IRGs), chemokine receptors, and other genes that participate in phagocytosis and leukocyte transendothelial migration. During infection with SARS-CoV not only a variety of IRGs were upregulated, but also a significantly broader range of genes involved in cell migration and inflammation. This work allowed dissection of several molecular signatures present during SARS-CoV which are part of a robust IFN antiviral response. These signatures can be useful markers to evaluate the status of IFN responses during a viral infection and specific features of different viruses.

Original languageEnglish (US)
Pages (from-to)102-112
Number of pages11
JournalVirology
Volume409
Issue number1
DOIs
StatePublished - Jan 5 2011
Externally publishedYes

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Coronavirus Infections
SARS Virus
Ferrets
Interferons
Gene Expression
Genes
Virus Diseases
Viruses
Transendothelial and Transepithelial Migration
Interferon Type I
Chemokine Receptors
Infection
Phagocytosis
Cell Communication
Cell Movement
Antiviral Agents
Dissection
Leukocytes
Up-Regulation
Phosphorylation

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

Early gene expression events in ferrets in response to SARS coronavirus infection versus direct interferon-alpha2b stimulation. / Danesh, Ali; Cameron, Cheryl M.; León, Alberto J.; Ran, Longsi; Xu, Luoling; Fang, Yuan; Kelvin, Alyson A.; Rowe, Thomas; Chen, Honglin; Guan, Yi; Jonsson, Colleen; Cameron, Mark J.; Kelvin, David J.

In: Virology, Vol. 409, No. 1, 05.01.2011, p. 102-112.

Research output: Contribution to journalArticle

Danesh, A, Cameron, CM, León, AJ, Ran, L, Xu, L, Fang, Y, Kelvin, AA, Rowe, T, Chen, H, Guan, Y, Jonsson, C, Cameron, MJ & Kelvin, DJ 2011, 'Early gene expression events in ferrets in response to SARS coronavirus infection versus direct interferon-alpha2b stimulation', Virology, vol. 409, no. 1, pp. 102-112. https://doi.org/10.1016/j.virol.2010.10.002
Danesh, Ali ; Cameron, Cheryl M. ; León, Alberto J. ; Ran, Longsi ; Xu, Luoling ; Fang, Yuan ; Kelvin, Alyson A. ; Rowe, Thomas ; Chen, Honglin ; Guan, Yi ; Jonsson, Colleen ; Cameron, Mark J. ; Kelvin, David J. / Early gene expression events in ferrets in response to SARS coronavirus infection versus direct interferon-alpha2b stimulation. In: Virology. 2011 ; Vol. 409, No. 1. pp. 102-112.
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