Editing and escape from editing in anti-DNA B cells

Salar N. Khan, Esther J. Witsch, Noah G. Goodman, Anil K. Panigrahi, Ching Chen, Yufei Jiang, Amy M. Cline, Jan Erikson, Martin Weigert, Eline T. Luning Prak, Marko Radic

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Tolerance to dsDNA is achieved through editing of Ig receptors that react with dsDNA. Nevertheless, some B cells with anti-dsDNA receptors escape editing and migrate to the spleen. Certain anti-dsDNA B cells that are recovered as hybridomas from the spleens of anti-dsDNA H chain transgenic mice also bind an additional, Golgi-associated antigen. B cells that bind this antigen accumulate intracellular IgM. The intracellular accumulation of IgM is incomplete, because IgM clusters are observed at the cell surface. In the spleen, B cells that express the heavy and light chains encoding this IgM are surface IgM-bright and acquire the CD21-high/CD23-low phenotype of marginal zone B cells. Our data imply that expression of an Ig that binds dsDNA and an additional antigen expressed in the secretory compartment renders B cells resistant to central tolerance. In the periphery, these B cells may be sequestered in the splenic marginal zone.

Original languageEnglish (US)
Pages (from-to)3861-3866
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number10
DOIs
StatePublished - Mar 25 2008

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B-Lymphocytes
Immunoglobulin M
DNA
Spleen
Antigens
Central Tolerance
Hybridomas
Transgenic Mice
Phenotype
Light

All Science Journal Classification (ASJC) codes

  • General

Cite this

Editing and escape from editing in anti-DNA B cells. / Khan, Salar N.; Witsch, Esther J.; Goodman, Noah G.; Panigrahi, Anil K.; Chen, Ching; Jiang, Yufei; Cline, Amy M.; Erikson, Jan; Weigert, Martin; Luning Prak, Eline T.; Radic, Marko.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, No. 10, 25.03.2008, p. 3861-3866.

Research output: Contribution to journalArticle

Khan, SN, Witsch, EJ, Goodman, NG, Panigrahi, AK, Chen, C, Jiang, Y, Cline, AM, Erikson, J, Weigert, M, Luning Prak, ET & Radic, M 2008, 'Editing and escape from editing in anti-DNA B cells', Proceedings of the National Academy of Sciences of the United States of America, vol. 105, no. 10, pp. 3861-3866. https://doi.org/10.1073/pnas.0800025105
Khan, Salar N. ; Witsch, Esther J. ; Goodman, Noah G. ; Panigrahi, Anil K. ; Chen, Ching ; Jiang, Yufei ; Cline, Amy M. ; Erikson, Jan ; Weigert, Martin ; Luning Prak, Eline T. ; Radic, Marko. / Editing and escape from editing in anti-DNA B cells. In: Proceedings of the National Academy of Sciences of the United States of America. 2008 ; Vol. 105, No. 10. pp. 3861-3866.
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