Effect of 15-HETE on cerebral arterioles of newborn pigs

G. K. Lovelady, R. Mirro, W. M. Armstead, D. W. Busija, Charles Leffler

Research output: Contribution to journalArticle

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Abstract

Effects of topical application of 15-HETE on pial arteriolar diameter and cortical perirachnoid cerebrospinal fluid (CSF) prostanoid concentrations were investigated in chloralose-anesthetized newborn pigs. Pial arteriolar diameters were measured using a closed cranial window, and CSF samples from under the window were collected for prostanoid analysis after applying artificial CSF without drug and CSF containing 15-HETE (1, 10, 100, 1000 ng/ml). 15-HETE caused significant dose-related constriction from 162 ± 17.0 μm (control diameter) to 136 ± 14.5 and 129 ± 18.7 μm (100 and 1000 ng/ml, respectively). The concentration of PGE2 (but not of PGF or 6-keto-PGF increased in CSF at 100 and 1000 ng/ml of 15-HETE. Pial arteriolar responses to 15-HETE were determined before and after indomethacin treatment (5 mg/kg, i.v.). 15-HETE (100 ng/ml) constricted pial arterioles before indomethacin (diameter change, -15 ± 10%); after indomethacin, constriction was potentiated in response to the same dose (diameter change, -26 ± 7%). These data support the hypothesis thet, in newborn piglets, 15-HETE exerts a vasoconstrictor effect on pial arterioles, which appears to be attenuated by 15-HETE-induced stimulation of dilator prostanoids.

Original languageEnglish (US)
Pages (from-to)507-513
Number of pages7
JournalProstaglandins
Volume36
Issue number4
DOIs
StatePublished - Jan 1 1988

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Arterioles
Cerebrospinal fluid
Swine
Cerebrospinal Fluid
Indomethacin
Prostaglandins
Constriction
Chloralose
Dinoprost
15-hydroxy-5,8,11,13-eicosatetraenoic acid
Vasoconstrictor Agents
Dinoprostone
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Endocrinology

Cite this

Lovelady, G. K., Mirro, R., Armstead, W. M., Busija, D. W., & Leffler, C. (1988). Effect of 15-HETE on cerebral arterioles of newborn pigs. Prostaglandins, 36(4), 507-513. https://doi.org/10.1016/0090-6980(88)90046-9

Effect of 15-HETE on cerebral arterioles of newborn pigs. / Lovelady, G. K.; Mirro, R.; Armstead, W. M.; Busija, D. W.; Leffler, Charles.

In: Prostaglandins, Vol. 36, No. 4, 01.01.1988, p. 507-513.

Research output: Contribution to journalArticle

Lovelady, GK, Mirro, R, Armstead, WM, Busija, DW & Leffler, C 1988, 'Effect of 15-HETE on cerebral arterioles of newborn pigs', Prostaglandins, vol. 36, no. 4, pp. 507-513. https://doi.org/10.1016/0090-6980(88)90046-9
Lovelady, G. K. ; Mirro, R. ; Armstead, W. M. ; Busija, D. W. ; Leffler, Charles. / Effect of 15-HETE on cerebral arterioles of newborn pigs. In: Prostaglandins. 1988 ; Vol. 36, No. 4. pp. 507-513.
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