[Effect of 17beta-estradiol on phosphorylcholine cytidylyltransferase activity from cultured rat lung explants].

Cha Xiang Guan, Fu Wen Zhou, Zi Qiang Luo, Chang Qing Zhang, Xiao Qun Qin

Research output: Contribution to journalArticle

Abstract

To investigate the influence and mechanisms of 17beta-estradiol on the CTP: phosphorylcholine cytidylyltransferase (CCT) activity from cultured lung explants without serum. We detected the amount of [M-14C] choline incorporation into phosphatidylcholine so as to reflect CCT activity by liquid scintillation. (1) 17beta-estradiol increased the CCT activity in dose-dependence and time-dependence. (2) Both the protein kinase C inhibitor H-7 and calmodulin antagonist W-7 abolished the stimulatory effect of 17beta-estradiol (3 x 10(-6) mol/L) on the CCT activity. 17beta-estradiol can increase CCT activity in cultured lung explants, its mechanism is related to protein kinase C and calmodulin.

Original languageEnglish (US)
Pages (from-to)57-59
Number of pages3
JournalZhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology
Volume19
Issue number1
StatePublished - Feb 1 2003

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Cytidine Triphosphate
Phosphorylcholine
Estradiol
Lung
Calmodulin
Protein Kinase C
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
Protein C Inhibitor
Protein Kinase Inhibitors
Choline
Phosphatidylcholines
Serum

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

[Effect of 17beta-estradiol on phosphorylcholine cytidylyltransferase activity from cultured rat lung explants]. / Guan, Cha Xiang; Zhou, Fu Wen; Luo, Zi Qiang; Zhang, Chang Qing; Qin, Xiao Qun.

In: Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology, Vol. 19, No. 1, 01.02.2003, p. 57-59.

Research output: Contribution to journalArticle

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