Effect of benzyl isothiocyanate on spontaneous and induced force of rat uterine contraction

Adebowale Adebiyi, P. Ganesan Adaikan, R. N.V. Prasad

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The present study examines the effect of benzyl isothiocyanate (BITC) on uterine contraction in vitro. BITC (10-320μM) caused irreversible, concentration-dependent inhibition of the spontaneous, prostaglandin F (PGF ) and oxytocin-induced force of gravid and non-gravid rat uterine contractions in contrast to equivalent concentrations of DMSO (solvent control). At 160μM of BITC, spontaneous, PGF and oxytocin-induced force of gravid rat myometrial contractions were reduced to 16±6%, 15±7% and 17±4% (of the control contractions), respectively. Moreover, at 320μM of BITC, spontaneous, PGF and oxytocin-induced force of non-gravid rat uterine contractions were reduced to 10±5%, 4±1% and 7±2% (of the control contractions), respectively. Incubation of isolated non-gravid rat uterine strips in Ringer Locke solution containing 100μM of BITC for 1h prior to recording their activity also caused significant and irreversible depression of KCl (60mM)-induced tension development in the uterus relative to the solvent control (P<0.01). In 56% of BITC-pretreated uterine tissues, spontaneous contractions were totally abolished. Cryosections of BITC-treated uterus (hematoxyline and eosin stained) examined under light microscope revealed structural disintegrity with marked vacuolar degeneration of the endometrium and myometrium. It thus appears that like the vascular smooth muscle (reported by previous workers), BITC is also capable of causing functional aberration of isolated uterus by provoking degeneration of the myometrium.

Original languageEnglish (US)
Pages (from-to)415-422
Number of pages8
JournalPharmacological Research
Volume49
Issue number5
DOIs
StatePublished - May 1 2004
Externally publishedYes

Fingerprint

Uterine Contraction
Prostaglandins F
Oxytocin
Uterus
Myometrium
benzyl isothiocyanate
Eosine Yellowish-(YS)
Endometrium
Dimethyl Sulfoxide
Vascular Smooth Muscle
Light

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

Effect of benzyl isothiocyanate on spontaneous and induced force of rat uterine contraction. / Adebiyi, Adebowale; Adaikan, P. Ganesan; Prasad, R. N.V.

In: Pharmacological Research, Vol. 49, No. 5, 01.05.2004, p. 415-422.

Research output: Contribution to journalArticle

@article{1b2e3d5f69624a04b61c5ffbc001b4c9,
title = "Effect of benzyl isothiocyanate on spontaneous and induced force of rat uterine contraction",
abstract = "The present study examines the effect of benzyl isothiocyanate (BITC) on uterine contraction in vitro. BITC (10-320μM) caused irreversible, concentration-dependent inhibition of the spontaneous, prostaglandin F 2α (PGF 2α ) and oxytocin-induced force of gravid and non-gravid rat uterine contractions in contrast to equivalent concentrations of DMSO (solvent control). At 160μM of BITC, spontaneous, PGF 2α and oxytocin-induced force of gravid rat myometrial contractions were reduced to 16±6{\%}, 15±7{\%} and 17±4{\%} (of the control contractions), respectively. Moreover, at 320μM of BITC, spontaneous, PGF 2α and oxytocin-induced force of non-gravid rat uterine contractions were reduced to 10±5{\%}, 4±1{\%} and 7±2{\%} (of the control contractions), respectively. Incubation of isolated non-gravid rat uterine strips in Ringer Locke solution containing 100μM of BITC for 1h prior to recording their activity also caused significant and irreversible depression of KCl (60mM)-induced tension development in the uterus relative to the solvent control (P<0.01). In 56{\%} of BITC-pretreated uterine tissues, spontaneous contractions were totally abolished. Cryosections of BITC-treated uterus (hematoxyline and eosin stained) examined under light microscope revealed structural disintegrity with marked vacuolar degeneration of the endometrium and myometrium. It thus appears that like the vascular smooth muscle (reported by previous workers), BITC is also capable of causing functional aberration of isolated uterus by provoking degeneration of the myometrium.",
author = "Adebowale Adebiyi and Adaikan, {P. Ganesan} and Prasad, {R. N.V.}",
year = "2004",
month = "5",
day = "1",
doi = "10.1016/j.phrs.2003.10.011",
language = "English (US)",
volume = "49",
pages = "415--422",
journal = "Pharmacological Research",
issn = "1043-6618",
publisher = "Academic Press Inc.",
number = "5",

}

TY - JOUR

T1 - Effect of benzyl isothiocyanate on spontaneous and induced force of rat uterine contraction

AU - Adebiyi, Adebowale

AU - Adaikan, P. Ganesan

AU - Prasad, R. N.V.

PY - 2004/5/1

Y1 - 2004/5/1

N2 - The present study examines the effect of benzyl isothiocyanate (BITC) on uterine contraction in vitro. BITC (10-320μM) caused irreversible, concentration-dependent inhibition of the spontaneous, prostaglandin F 2α (PGF 2α ) and oxytocin-induced force of gravid and non-gravid rat uterine contractions in contrast to equivalent concentrations of DMSO (solvent control). At 160μM of BITC, spontaneous, PGF 2α and oxytocin-induced force of gravid rat myometrial contractions were reduced to 16±6%, 15±7% and 17±4% (of the control contractions), respectively. Moreover, at 320μM of BITC, spontaneous, PGF 2α and oxytocin-induced force of non-gravid rat uterine contractions were reduced to 10±5%, 4±1% and 7±2% (of the control contractions), respectively. Incubation of isolated non-gravid rat uterine strips in Ringer Locke solution containing 100μM of BITC for 1h prior to recording their activity also caused significant and irreversible depression of KCl (60mM)-induced tension development in the uterus relative to the solvent control (P<0.01). In 56% of BITC-pretreated uterine tissues, spontaneous contractions were totally abolished. Cryosections of BITC-treated uterus (hematoxyline and eosin stained) examined under light microscope revealed structural disintegrity with marked vacuolar degeneration of the endometrium and myometrium. It thus appears that like the vascular smooth muscle (reported by previous workers), BITC is also capable of causing functional aberration of isolated uterus by provoking degeneration of the myometrium.

AB - The present study examines the effect of benzyl isothiocyanate (BITC) on uterine contraction in vitro. BITC (10-320μM) caused irreversible, concentration-dependent inhibition of the spontaneous, prostaglandin F 2α (PGF 2α ) and oxytocin-induced force of gravid and non-gravid rat uterine contractions in contrast to equivalent concentrations of DMSO (solvent control). At 160μM of BITC, spontaneous, PGF 2α and oxytocin-induced force of gravid rat myometrial contractions were reduced to 16±6%, 15±7% and 17±4% (of the control contractions), respectively. Moreover, at 320μM of BITC, spontaneous, PGF 2α and oxytocin-induced force of non-gravid rat uterine contractions were reduced to 10±5%, 4±1% and 7±2% (of the control contractions), respectively. Incubation of isolated non-gravid rat uterine strips in Ringer Locke solution containing 100μM of BITC for 1h prior to recording their activity also caused significant and irreversible depression of KCl (60mM)-induced tension development in the uterus relative to the solvent control (P<0.01). In 56% of BITC-pretreated uterine tissues, spontaneous contractions were totally abolished. Cryosections of BITC-treated uterus (hematoxyline and eosin stained) examined under light microscope revealed structural disintegrity with marked vacuolar degeneration of the endometrium and myometrium. It thus appears that like the vascular smooth muscle (reported by previous workers), BITC is also capable of causing functional aberration of isolated uterus by provoking degeneration of the myometrium.

UR - http://www.scopus.com/inward/record.url?scp=1442333594&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1442333594&partnerID=8YFLogxK

U2 - 10.1016/j.phrs.2003.10.011

DO - 10.1016/j.phrs.2003.10.011

M3 - Article

VL - 49

SP - 415

EP - 422

JO - Pharmacological Research

JF - Pharmacological Research

SN - 1043-6618

IS - 5

ER -