Effect of cyclic 3′,5′-guanosine monophosphate on human platelet function

T. M. Chiang, S. N. Dixit, Andrew Kang

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The relation of cyclic 3′,5′-guanosine monophosphate (cGMP) to platelet function was studied by investigating the influence of this compound and its N2,O2′-dibutyryl derivative (DBcGMP) on platelet aggregation and the release reaction. Both cGMP and dibutyryl cGMP enhanced to an equal extent platelet aggregation induced by epinephrine or by chick skin collagen α1 chain. The platelet release reaction, as measured by the release of [14C]-labeled 5-hydroxytryptamine (serotonin) was also enhanced by the cyclic nucleotides. Both compounds were also able to partially overcome the inhibitory effect of N6,O2′-dibutyryl 3′,5′-adenosine monophosphate (DBcAMP), prostaglandin E1, and theophylline. The effect of DBcGMP did not result from contamination with 5′-GMP. Butyric acid and 5′-GMP, either alone or in combination, had no detectable effect on platelet function. The in vitro effect of DBcGMP and cGMP was not dependent on preincubation of platelets with the compounds. This suggests that their effect is mediated by direct action on the platelet membranes. These data are consistent with previous observations that the platelet aggregating agents (epinephrine, ADP, collagen, and chick collagen α1 chains) cause an increase in the content of GMP, and support the hypothesis that platelet aggregation is favored by an increase in cGMP.

Original languageEnglish (US)
Pages (from-to)215-221
Number of pages7
JournalThe Journal of Laboratory and Clinical Medicine
Volume88
Issue number2
StatePublished - Jan 1 1976

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Platelets
Blood Platelets
Platelet Aggregation
Guanosine Monophosphate
Collagen
Epinephrine
Serotonin
Agglomeration
Bucladesine
Butyric Acid
Alprostadil
Cyclic Nucleotides
Adenosine Monophosphate
Theophylline
Adenosine Diphosphate
3'-guanylic acid
Cyclic GMP
Skin
Membranes
Contamination

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Effect of cyclic 3′,5′-guanosine monophosphate on human platelet function. / Chiang, T. M.; Dixit, S. N.; Kang, Andrew.

In: The Journal of Laboratory and Clinical Medicine, Vol. 88, No. 2, 01.01.1976, p. 215-221.

Research output: Contribution to journalArticle

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