Effect of endosulfan on antioxidants of freshwater fish Channa punctatus bloch

1. Protection against lipid peroxidation in liver by copper preexposure

S. Pandey, I. Ahmad, S. Parvez, Bilal Hafeez, R. Haque, S. Raisuddin

Research output: Contribution to journalArticle

137 Citations (Scopus)

Abstract

Effect of a single exposure of endosulfan (5 ppb) on w catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), and reduced glutathione (GSH) of liver, kidney, and gill of a freshwater fish (Channa punctatus Bloch) were evaluated after 24 h of treatment. Endosulfan exposure resulted in a significant induction (p < 0.05-0.001) of GPx, GST activity, and GSH levels in all the organs. However, CAT activity was found to be significantly decreased (p < 0.010.001). Lipid peroxidation (LPO) values were also determined in liver, kidney, and gill and a significant increase in LPO values (p < 0.05-0.01) was observed in all the organs. We also investigated whether preexposure to low concentration of copper (10 ppb) for 4 weeks has any protective effect against endosulfan-induced oxidative damage. In copper-acclimatized endosulfan-exposed fish, a significant decrease in GPx (p < 0.001), GST (p < 0.05), GSH (p < 0.001) levels, and LPO (p < 0.01) was observed in the liver, whereas CAT activity was increased significantly (p < 0.001). However, kidney and gill did not show any significant alterations in antioxidant levels. The results of this study demonstrate that endosulfan induces peroxidative damage in liver, kidney, and gill in response to which levels of antioxidant were modulated. However, when fish preacclimatized to copper were exposed to endosulfan, protection against oxidative damage was observed only in the liver. It is proposed that measurement of antioxidants in fish tissues may prove to be useful in biomonitoring of exposure to aquatic pollutants.

Original languageEnglish (US)
Pages (from-to)345-352
Number of pages8
JournalArchives of Environmental Contamination and Toxicology
Volume41
Issue number3
DOIs
StatePublished - Jan 1 2001

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Endosulfan
Antioxidants
Fresh Water
Liver
Fish
Lipids
Lipid Peroxidation
Copper
Fishes
Glutathione Peroxidase
Glutathione Transferase
Catalase
Kidney
Environmental Monitoring
Glutathione
Tissue

All Science Journal Classification (ASJC) codes

  • Toxicology
  • Pollution
  • Health, Toxicology and Mutagenesis

Cite this

Effect of endosulfan on antioxidants of freshwater fish Channa punctatus bloch : 1. Protection against lipid peroxidation in liver by copper preexposure. / Pandey, S.; Ahmad, I.; Parvez, S.; Hafeez, Bilal; Haque, R.; Raisuddin, S.

In: Archives of Environmental Contamination and Toxicology, Vol. 41, No. 3, 01.01.2001, p. 345-352.

Research output: Contribution to journalArticle

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abstract = "Effect of a single exposure of endosulfan (5 ppb) on w catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), and reduced glutathione (GSH) of liver, kidney, and gill of a freshwater fish (Channa punctatus Bloch) were evaluated after 24 h of treatment. Endosulfan exposure resulted in a significant induction (p < 0.05-0.001) of GPx, GST activity, and GSH levels in all the organs. However, CAT activity was found to be significantly decreased (p < 0.010.001). Lipid peroxidation (LPO) values were also determined in liver, kidney, and gill and a significant increase in LPO values (p < 0.05-0.01) was observed in all the organs. We also investigated whether preexposure to low concentration of copper (10 ppb) for 4 weeks has any protective effect against endosulfan-induced oxidative damage. In copper-acclimatized endosulfan-exposed fish, a significant decrease in GPx (p < 0.001), GST (p < 0.05), GSH (p < 0.001) levels, and LPO (p < 0.01) was observed in the liver, whereas CAT activity was increased significantly (p < 0.001). However, kidney and gill did not show any significant alterations in antioxidant levels. The results of this study demonstrate that endosulfan induces peroxidative damage in liver, kidney, and gill in response to which levels of antioxidant were modulated. However, when fish preacclimatized to copper were exposed to endosulfan, protection against oxidative damage was observed only in the liver. It is proposed that measurement of antioxidants in fish tissues may prove to be useful in biomonitoring of exposure to aquatic pollutants.",
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