Effect of endotoxic shock on basal and insulin-mediated Na+/K+-pump activity in rat soleus muscle

Michael Karlstad, M. M. Sayeed

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

This study evaluated basal and insulin-mediated Na+/K+ transport in skeletal muscle during endotoxic shock. Fasted male Holtzman rats (80-100 g) were killed 5 hr after the i.v. injection of saline (control) or 20 mg/kg Salmonella enteritidis endotoxin. 86Rb+ uptake into the cellular compartment of soleus muscle was determined in the presence and absence of 100 mU/ml insulin. The rate coefficient and rate of 22Na+ efflux and muscle intracellular 22Na+ content (in percentage of total muscle 22Na+) were determined in the presence and absence of 1 mM ouabain. The rate of basal cellular 86Rb+ uptake (cpm x 103 · g wet weight-1 · min-1) was not significantly different between control (77.7 ± 2.9) and endotoxic soleus muscles (76.1 ± 3.9). Insulin increased the rate of cellular 86Rb+ uptake by 19% in control (92.4 ± 3.7) and 23% in endotoxic soleus muscles (93.8 ± 4.1). Cellular 22Na+ content was 40% greater in endotoxic muscle (16.8 ± 1.4) as compared to control muscles (12.0 ± 0.9). The rate coefficient for ouabain-sensitive 22Na+ efflux in endotoxic muscles (0.028 min-1) was 1.7-fold greater than that in control muscles (0.017 min-1). The rate of ouabain-sensitive 22Na+ efflux was more than doubled in endotoxic muscles (0.470%/min) compared with control muscles (0.204%/min). Endotoxic shock did not alter insulin's ability to stimulate Na+/K+ transport in muscle. An increase in the electrogenicity of the Na+/K+ pump is consistent with an increased rate coefficient for 22Na+ efflux, with no change in the rate of 86Rb+ uptake in endotoxic soleus muscle. The paradoxical increase of cellular 22Na+ with greater electrogenic Na+/K+ transport may be due to increased cell membrane permeability to Na+ relative to K+ in endotoxic muscle.

Original languageEnglish (US)
Pages (from-to)222-227
Number of pages6
JournalCirculatory Shock
Volume38
Issue number4
StatePublished - 1992

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Septic Shock
Skeletal Muscle
Insulin
Muscles
Ouabain
Cell Membrane Permeability
Salmonella enteritidis
Sprague Dawley Rats
Weights and Measures
Injections

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

Cite this

Effect of endotoxic shock on basal and insulin-mediated Na+/K+-pump activity in rat soleus muscle. / Karlstad, Michael; Sayeed, M. M.

In: Circulatory Shock, Vol. 38, No. 4, 1992, p. 222-227.

Research output: Contribution to journalArticle

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abstract = "This study evaluated basal and insulin-mediated Na+/K+ transport in skeletal muscle during endotoxic shock. Fasted male Holtzman rats (80-100 g) were killed 5 hr after the i.v. injection of saline (control) or 20 mg/kg Salmonella enteritidis endotoxin. 86Rb+ uptake into the cellular compartment of soleus muscle was determined in the presence and absence of 100 mU/ml insulin. The rate coefficient and rate of 22Na+ efflux and muscle intracellular 22Na+ content (in percentage of total muscle 22Na+) were determined in the presence and absence of 1 mM ouabain. The rate of basal cellular 86Rb+ uptake (cpm x 103 · g wet weight-1 · min-1) was not significantly different between control (77.7 ± 2.9) and endotoxic soleus muscles (76.1 ± 3.9). Insulin increased the rate of cellular 86Rb+ uptake by 19{\%} in control (92.4 ± 3.7) and 23{\%} in endotoxic soleus muscles (93.8 ± 4.1). Cellular 22Na+ content was 40{\%} greater in endotoxic muscle (16.8 ± 1.4) as compared to control muscles (12.0 ± 0.9). The rate coefficient for ouabain-sensitive 22Na+ efflux in endotoxic muscles (0.028 min-1) was 1.7-fold greater than that in control muscles (0.017 min-1). The rate of ouabain-sensitive 22Na+ efflux was more than doubled in endotoxic muscles (0.470{\%}/min) compared with control muscles (0.204{\%}/min). Endotoxic shock did not alter insulin's ability to stimulate Na+/K+ transport in muscle. An increase in the electrogenicity of the Na+/K+ pump is consistent with an increased rate coefficient for 22Na+ efflux, with no change in the rate of 86Rb+ uptake in endotoxic soleus muscle. The paradoxical increase of cellular 22Na+ with greater electrogenic Na+/K+ transport may be due to increased cell membrane permeability to Na+ relative to K+ in endotoxic muscle.",
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