Effect of endotoxic shock on skeletal muscle intracellular electrolytes and amino acid transport

Michael Karlstad, M. M. Sayeed

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Abstract

Soleus muscle intracellular electrolytes and α-aminoisobutyric acid (AIB) uptake and its regulation by insulin were investigated during endotoxic shock in vivo. Fasted rats (90 g) were injected with [14C]AIB (1-10 mg/kg, iv) and Salmonella enteritidis endotoxin (20 mg/kg, iv) or saline and killed 1-5 h later. AIB uptake into muscle was corrected for uptake into extracellular space (measured as inulin distribution in muscle in vivo) and expressed as [AIB]/[AIB] ratios to asssess active transport. The maximum level of active AIB cell transport by extracellular endotoxic muscles [3.3 ± 0.2 (SE)] was lower than control muscles (4.9 ± 0.3) at the time when plasma insulin concentration in endotoxic rats (16.8 ± 2.0 uU/ml) was greater than controls (7.9 ± 1.7 uU/ml). Insulin-stimulated AIB transport was significantly lower in endotoxic muscles (from a basal value of 3.3 ± 0.2 to 4.4 ± 0.5, 5.0 ± 0.3, and 4.9 ± 0.3 at 125, 250, and 500 mU/kg intravenous insulin, respectively) than in control muscles (from 4.9 ± 0.3 to 5.7 ± 0.2, 8.0 ± 0.4, and 10.8 ± 0.8). The intracellular electrolyte concentrations in endotoxic soleus muscles ([Na] = 15.87 ± 0.9, [K] = 145 ± 1.7, [Cl] = 14.61 ± 0.9) were substantially altered when compared with control muscles ([Na] = 9.33 ± 1.1, [K] = 164 ± 1.5, [Cl] = 4.1 ± 0.6) 5 h postinjection. The membrane potential, estimated from cloride equilibrium potential was lower in endotoxic muscles (- 52.7 ± 1.6 mV) when compared with control muscles (- 89 ± 3.5 mV). The attenuation of AIB transport in endotoxic muscles may be due to decreased Na+ electrochemical energy. Alterations of basal and insulin-stimulated amino acid transport could adversely affect the size and composition of intracellular amino acid pools in skeletal muscle during endotoxic shock.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume252
Issue number4
StatePublished - 1987
Externally publishedYes

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Aminoisobutyric Acids
Septic Shock
Electrolytes
Skeletal Muscle
Amino Acids
Muscles
Insulin
Salmonella enteritidis
Inulin
Active Biological Transport
Extracellular Space
Membrane Potentials

All Science Journal Classification (ASJC) codes

  • Physiology

Cite this

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title = "Effect of endotoxic shock on skeletal muscle intracellular electrolytes and amino acid transport",
abstract = "Soleus muscle intracellular electrolytes and α-aminoisobutyric acid (AIB) uptake and its regulation by insulin were investigated during endotoxic shock in vivo. Fasted rats (90 g) were injected with [14C]AIB (1-10 mg/kg, iv) and Salmonella enteritidis endotoxin (20 mg/kg, iv) or saline and killed 1-5 h later. AIB uptake into muscle was corrected for uptake into extracellular space (measured as inulin distribution in muscle in vivo) and expressed as [AIB]/[AIB] ratios to asssess active transport. The maximum level of active AIB cell transport by extracellular endotoxic muscles [3.3 ± 0.2 (SE)] was lower than control muscles (4.9 ± 0.3) at the time when plasma insulin concentration in endotoxic rats (16.8 ± 2.0 uU/ml) was greater than controls (7.9 ± 1.7 uU/ml). Insulin-stimulated AIB transport was significantly lower in endotoxic muscles (from a basal value of 3.3 ± 0.2 to 4.4 ± 0.5, 5.0 ± 0.3, and 4.9 ± 0.3 at 125, 250, and 500 mU/kg intravenous insulin, respectively) than in control muscles (from 4.9 ± 0.3 to 5.7 ± 0.2, 8.0 ± 0.4, and 10.8 ± 0.8). The intracellular electrolyte concentrations in endotoxic soleus muscles ([Na] = 15.87 ± 0.9, [K] = 145 ± 1.7, [Cl] = 14.61 ± 0.9) were substantially altered when compared with control muscles ([Na] = 9.33 ± 1.1, [K] = 164 ± 1.5, [Cl] = 4.1 ± 0.6) 5 h postinjection. The membrane potential, estimated from cloride equilibrium potential was lower in endotoxic muscles (- 52.7 ± 1.6 mV) when compared with control muscles (- 89 ± 3.5 mV). The attenuation of AIB transport in endotoxic muscles may be due to decreased Na+ electrochemical energy. Alterations of basal and insulin-stimulated amino acid transport could adversely affect the size and composition of intracellular amino acid pools in skeletal muscle during endotoxic shock.",
author = "Michael Karlstad and Sayeed, {M. M.}",
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journal = "American Journal of Physiology",
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T1 - Effect of endotoxic shock on skeletal muscle intracellular electrolytes and amino acid transport

AU - Karlstad, Michael

AU - Sayeed, M. M.

PY - 1987

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N2 - Soleus muscle intracellular electrolytes and α-aminoisobutyric acid (AIB) uptake and its regulation by insulin were investigated during endotoxic shock in vivo. Fasted rats (90 g) were injected with [14C]AIB (1-10 mg/kg, iv) and Salmonella enteritidis endotoxin (20 mg/kg, iv) or saline and killed 1-5 h later. AIB uptake into muscle was corrected for uptake into extracellular space (measured as inulin distribution in muscle in vivo) and expressed as [AIB]/[AIB] ratios to asssess active transport. The maximum level of active AIB cell transport by extracellular endotoxic muscles [3.3 ± 0.2 (SE)] was lower than control muscles (4.9 ± 0.3) at the time when plasma insulin concentration in endotoxic rats (16.8 ± 2.0 uU/ml) was greater than controls (7.9 ± 1.7 uU/ml). Insulin-stimulated AIB transport was significantly lower in endotoxic muscles (from a basal value of 3.3 ± 0.2 to 4.4 ± 0.5, 5.0 ± 0.3, and 4.9 ± 0.3 at 125, 250, and 500 mU/kg intravenous insulin, respectively) than in control muscles (from 4.9 ± 0.3 to 5.7 ± 0.2, 8.0 ± 0.4, and 10.8 ± 0.8). The intracellular electrolyte concentrations in endotoxic soleus muscles ([Na] = 15.87 ± 0.9, [K] = 145 ± 1.7, [Cl] = 14.61 ± 0.9) were substantially altered when compared with control muscles ([Na] = 9.33 ± 1.1, [K] = 164 ± 1.5, [Cl] = 4.1 ± 0.6) 5 h postinjection. The membrane potential, estimated from cloride equilibrium potential was lower in endotoxic muscles (- 52.7 ± 1.6 mV) when compared with control muscles (- 89 ± 3.5 mV). The attenuation of AIB transport in endotoxic muscles may be due to decreased Na+ electrochemical energy. Alterations of basal and insulin-stimulated amino acid transport could adversely affect the size and composition of intracellular amino acid pools in skeletal muscle during endotoxic shock.

AB - Soleus muscle intracellular electrolytes and α-aminoisobutyric acid (AIB) uptake and its regulation by insulin were investigated during endotoxic shock in vivo. Fasted rats (90 g) were injected with [14C]AIB (1-10 mg/kg, iv) and Salmonella enteritidis endotoxin (20 mg/kg, iv) or saline and killed 1-5 h later. AIB uptake into muscle was corrected for uptake into extracellular space (measured as inulin distribution in muscle in vivo) and expressed as [AIB]/[AIB] ratios to asssess active transport. The maximum level of active AIB cell transport by extracellular endotoxic muscles [3.3 ± 0.2 (SE)] was lower than control muscles (4.9 ± 0.3) at the time when plasma insulin concentration in endotoxic rats (16.8 ± 2.0 uU/ml) was greater than controls (7.9 ± 1.7 uU/ml). Insulin-stimulated AIB transport was significantly lower in endotoxic muscles (from a basal value of 3.3 ± 0.2 to 4.4 ± 0.5, 5.0 ± 0.3, and 4.9 ± 0.3 at 125, 250, and 500 mU/kg intravenous insulin, respectively) than in control muscles (from 4.9 ± 0.3 to 5.7 ± 0.2, 8.0 ± 0.4, and 10.8 ± 0.8). The intracellular electrolyte concentrations in endotoxic soleus muscles ([Na] = 15.87 ± 0.9, [K] = 145 ± 1.7, [Cl] = 14.61 ± 0.9) were substantially altered when compared with control muscles ([Na] = 9.33 ± 1.1, [K] = 164 ± 1.5, [Cl] = 4.1 ± 0.6) 5 h postinjection. The membrane potential, estimated from cloride equilibrium potential was lower in endotoxic muscles (- 52.7 ± 1.6 mV) when compared with control muscles (- 89 ± 3.5 mV). The attenuation of AIB transport in endotoxic muscles may be due to decreased Na+ electrochemical energy. Alterations of basal and insulin-stimulated amino acid transport could adversely affect the size and composition of intracellular amino acid pools in skeletal muscle during endotoxic shock.

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