Effect of exercise on biological pathways in ApcMin/+ mouse intestinal polyps

Kristen A. Baltgalvis, Franklin G. Berger, Maria Marjorette O. Peña, J. Mark Davis, James Carson

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Many epidemiological studies have demonstrated that level of exercise is associated with reduced colorectal cancer risk. Treadmill training can decrease ApcMin/+ mouse intestinal polyp number and size, but the mechanisms remain unclear. Understanding the molecular changes in the tumor following exercise training may provide insight on the mechanism by which exercise decreases ApcMin/+ mouse polyp formation and growth. The purpose of this study was to determine if exercise can modulate ApcMin/+ mouse intestinal polyp cellular signaling related to tumor formation and growth. Male ApcMin/+ mice were randomly assigned to control (n = 20) or exercise (n = 20) treatment groups. Exercised mice ran on a treadmill at a moderate intensity (18 m/min, 60 min, 6 days/wk, 5% grade) for 9 wk. Polyps from Apc Min/+ mice were used to quantify markers of polyp inflammation, apoptosis, and β-catenin signaling. Exercise decreased the number of macrophages in polyps by 35%. Related to apoptosis, exercise decreased the number of terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL)-positive cells by 73% in all polyps. Bax protein expression in polyps was decreased 43% by exercise. β-Catenin phosphorylation was elevated 3.3-fold in polyps from exercised mice. Moderate-intensity exercise training alters cellular pathways in ApcMin/+ mouse polyps, and these changes may be related to the exercise-induced reduction in polyp formation and growth.

Original languageEnglish (US)
Pages (from-to)1137-1143
Number of pages7
JournalJournal of Applied Physiology
Volume104
Issue number4
DOIs
StatePublished - Apr 1 2008
Externally publishedYes

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Intestinal Polyps
Polyps
Catenins
Growth
Exercise
Apoptosis
bcl-2-Associated X Protein
DNA Nucleotidylexotransferase
Epidemiologic Studies
Colorectal Neoplasms
Neoplasms
Macrophages
Phosphorylation

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

Effect of exercise on biological pathways in ApcMin/+ mouse intestinal polyps. / Baltgalvis, Kristen A.; Berger, Franklin G.; Peña, Maria Marjorette O.; Davis, J. Mark; Carson, James.

In: Journal of Applied Physiology, Vol. 104, No. 4, 01.04.2008, p. 1137-1143.

Research output: Contribution to journalArticle

Baltgalvis, Kristen A. ; Berger, Franklin G. ; Peña, Maria Marjorette O. ; Davis, J. Mark ; Carson, James. / Effect of exercise on biological pathways in ApcMin/+ mouse intestinal polyps. In: Journal of Applied Physiology. 2008 ; Vol. 104, No. 4. pp. 1137-1143.
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