Effect of exposure to novelty on brain monoamines in C57BL/6 and DBA/2 mice

Byron Jones, Hou Xu, Melloni N. Cook

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Male and female mice from two inbred strains, C57BL/6 (B6) and DBA/2 (D2), were exposed to a novel environment (vs. undisturbed control) for 10 min. Immediately after this treatment, the animals were sacrificed by cervical dislocation, and the brains were removed and dissected into ventral midbrain (VMB), caudate-putamen (CP), nucleus accumbens (NA), and medial prefrontal cortex (FC). Analyses of dopamine (DA) and its metabolites, dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA) and serotonin (5-HT) and its metabolite, 5-hydroxyindole-3-acetic acid (5-HIAA) were made by high-performance liquid chromatography. Utilization of the parent amines was estimated by the ratios, DOPAC/DA, HVA/DA, and 5-HIAA/5-HT. Novelty increased DOPAC levels in NA of both strains of mice and in CP of D2 males; however, it did not induce significant changes in DA, or 5-HT levels or utilization of the latter. The results did, however, reveal large strain differences in DA and its metabolites. The data suggest that genetically based neurophysiological and neurochemical differences exist in dopaminergic and serotonergic systems in mice, and that the DA systems in NA may be more sensitive to novelty than other DA systems.

Original languageEnglish (US)
Pages (from-to)361-367
Number of pages7
JournalPhysiology and Behavior
Volume59
Issue number2
DOIs
StatePublished - Jan 1 1996

Fingerprint

Inbred DBA Mouse
Dopamine
Brain
Serotonin
Nucleus Accumbens
Homovanillic Acid
Putamen
Acetic Acid
Acids
Inbred Strains Mice
Caudate Nucleus
Mesencephalon
Prefrontal Cortex
Amines
High Pressure Liquid Chromatography

All Science Journal Classification (ASJC) codes

  • Experimental and Cognitive Psychology
  • Behavioral Neuroscience

Cite this

Effect of exposure to novelty on brain monoamines in C57BL/6 and DBA/2 mice. / Jones, Byron; Xu, Hou; Cook, Melloni N.

In: Physiology and Behavior, Vol. 59, No. 2, 01.01.1996, p. 361-367.

Research output: Contribution to journalArticle

Jones, Byron ; Xu, Hou ; Cook, Melloni N. / Effect of exposure to novelty on brain monoamines in C57BL/6 and DBA/2 mice. In: Physiology and Behavior. 1996 ; Vol. 59, No. 2. pp. 361-367.
@article{a9aa556d7d594922b289bc3de998b792,
title = "Effect of exposure to novelty on brain monoamines in C57BL/6 and DBA/2 mice",
abstract = "Male and female mice from two inbred strains, C57BL/6 (B6) and DBA/2 (D2), were exposed to a novel environment (vs. undisturbed control) for 10 min. Immediately after this treatment, the animals were sacrificed by cervical dislocation, and the brains were removed and dissected into ventral midbrain (VMB), caudate-putamen (CP), nucleus accumbens (NA), and medial prefrontal cortex (FC). Analyses of dopamine (DA) and its metabolites, dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA) and serotonin (5-HT) and its metabolite, 5-hydroxyindole-3-acetic acid (5-HIAA) were made by high-performance liquid chromatography. Utilization of the parent amines was estimated by the ratios, DOPAC/DA, HVA/DA, and 5-HIAA/5-HT. Novelty increased DOPAC levels in NA of both strains of mice and in CP of D2 males; however, it did not induce significant changes in DA, or 5-HT levels or utilization of the latter. The results did, however, reveal large strain differences in DA and its metabolites. The data suggest that genetically based neurophysiological and neurochemical differences exist in dopaminergic and serotonergic systems in mice, and that the DA systems in NA may be more sensitive to novelty than other DA systems.",
author = "Byron Jones and Hou Xu and Cook, {Melloni N.}",
year = "1996",
month = "1",
day = "1",
doi = "10.1016/0031-9384(95)02010-1",
language = "English (US)",
volume = "59",
pages = "361--367",
journal = "Physiology and Behavior",
issn = "0031-9384",
publisher = "Elsevier Inc.",
number = "2",

}

TY - JOUR

T1 - Effect of exposure to novelty on brain monoamines in C57BL/6 and DBA/2 mice

AU - Jones, Byron

AU - Xu, Hou

AU - Cook, Melloni N.

PY - 1996/1/1

Y1 - 1996/1/1

N2 - Male and female mice from two inbred strains, C57BL/6 (B6) and DBA/2 (D2), were exposed to a novel environment (vs. undisturbed control) for 10 min. Immediately after this treatment, the animals were sacrificed by cervical dislocation, and the brains were removed and dissected into ventral midbrain (VMB), caudate-putamen (CP), nucleus accumbens (NA), and medial prefrontal cortex (FC). Analyses of dopamine (DA) and its metabolites, dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA) and serotonin (5-HT) and its metabolite, 5-hydroxyindole-3-acetic acid (5-HIAA) were made by high-performance liquid chromatography. Utilization of the parent amines was estimated by the ratios, DOPAC/DA, HVA/DA, and 5-HIAA/5-HT. Novelty increased DOPAC levels in NA of both strains of mice and in CP of D2 males; however, it did not induce significant changes in DA, or 5-HT levels or utilization of the latter. The results did, however, reveal large strain differences in DA and its metabolites. The data suggest that genetically based neurophysiological and neurochemical differences exist in dopaminergic and serotonergic systems in mice, and that the DA systems in NA may be more sensitive to novelty than other DA systems.

AB - Male and female mice from two inbred strains, C57BL/6 (B6) and DBA/2 (D2), were exposed to a novel environment (vs. undisturbed control) for 10 min. Immediately after this treatment, the animals were sacrificed by cervical dislocation, and the brains were removed and dissected into ventral midbrain (VMB), caudate-putamen (CP), nucleus accumbens (NA), and medial prefrontal cortex (FC). Analyses of dopamine (DA) and its metabolites, dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA) and serotonin (5-HT) and its metabolite, 5-hydroxyindole-3-acetic acid (5-HIAA) were made by high-performance liquid chromatography. Utilization of the parent amines was estimated by the ratios, DOPAC/DA, HVA/DA, and 5-HIAA/5-HT. Novelty increased DOPAC levels in NA of both strains of mice and in CP of D2 males; however, it did not induce significant changes in DA, or 5-HT levels or utilization of the latter. The results did, however, reveal large strain differences in DA and its metabolites. The data suggest that genetically based neurophysiological and neurochemical differences exist in dopaminergic and serotonergic systems in mice, and that the DA systems in NA may be more sensitive to novelty than other DA systems.

UR - http://www.scopus.com/inward/record.url?scp=0343396498&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0343396498&partnerID=8YFLogxK

U2 - 10.1016/0031-9384(95)02010-1

DO - 10.1016/0031-9384(95)02010-1

M3 - Article

VL - 59

SP - 361

EP - 367

JO - Physiology and Behavior

JF - Physiology and Behavior

SN - 0031-9384

IS - 2

ER -