Effect of functional modulation of Ca 2+ -activated Cl - currents on gravid rat myometrial activity

P. Ganesan Adaikan, Adebowale Adebiyi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objective: This study was carried out to examine the effect of functional modification of Ca 2+ -activated Cl - currents on contractile activities of isolated gravid rat myometrium. Materials and Methods: Spontaneous rhythmic and oxytocin-induced contractile activities of gravid rat myometrium in the presence and absence of Cl - (in the bathing medium) were studied in a tissue bath system. The effects of niflumic acid (a Cl - channel blocker) on spontaneous and oxytocin-induced contractile activities were also delineated. Results: Replacement of Cl - - containing Tyrode solution with Cl - -free medium caused transient increase in myometrial contractile tone for about 15 min (possibly due to rapid efflux of Cl - ) after which gradual failure of the spontaneous contractions led to myometrial quiescence within 1 h. Normal spontaneous contractions returned after replacing Cl - -free medium with Tyrode solution. In Cl - -free medium, oxytocin-induced contractions of the myometrium were significantly reduced by 44 ± 9%. Niflumic acid (30 μM) caused an increase in myometrial contractile tone for about 10 min followed by time-dependent inhibition of spontaneous contractions of the rat myometrium. Moreover, prior addition of 30 μM of niflumic acid to the tissue baths (for 30 min) caused 74 ± 5% reduction of oxytocin-induced contractions of the myometrial strips. Conclusion: Ca 2+ -activated Cl - currents play a significant role in the spontaneous and oxytocin-induced myometrial activity. Ca 2+ -activated Cl - conductance could hence be a potential target for the pharmacological control of uterine activity.

Original languageEnglish (US)
Pages (from-to)21-25
Number of pages5
JournalIndian Journal of Pharmacology
Volume37
Issue number1
DOIs
StatePublished - Jan 1 2005

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Oxytocin
Myometrium
Niflumic Acid
Baths
Uterine Contraction
Pharmacology
Tyrode's solution

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)

Cite this

Effect of functional modulation of Ca 2+ -activated Cl - currents on gravid rat myometrial activity . / Adaikan, P. Ganesan; Adebiyi, Adebowale.

In: Indian Journal of Pharmacology, Vol. 37, No. 1, 01.01.2005, p. 21-25.

Research output: Contribution to journalArticle

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