Effect of intestinal chylomicron secretory blockade on apolipoprotein synthesis in the newborn piglet

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Abstract

Pluronic L-81 is a hydrophobic surfactant which blocks intestinal chylomicron secretion at the pre-Golgi level without affecting triacylglycerol uptake and re-esterification. To study the effects of such blockade on apolipoprotein synthesis, newborn female piglets received 24 h intraduodenal infusions of low-triacylglycerol, or high-triacylglycerol with or without Pluronic L-81, diets, followed by determination of apolipoprotein (apo) B-48, A-I and A-IV synthesis and content and apo B and A-IV mRNA levels in the small intestine. Jejunal apo B-48 content, synthesis and mRNA levels were down-regulated below basal levels by the addition of Pluronic to the high-triacylglycerol infusion. The normal increase in apo A-I synthesis induced by triacylglycerol absorption was ablated in both jejunum and ileum, even though the expected increase in apo A-I content in jejunum still occurred. Although attenuated, the expected increase in jejunal apo A-IV synthesis and mRNA levels with triacylglycerol absorption was still present with Pluronic treatment. These results suggest very different mechanisms of cellular regulation and trafficking for the various apolipoproteins incorporated into nascent intestinal chylomicrons. Apo B may be specifically down-regulated by the chylomicron secretory blockade induced by Pluronic L-81.

Original languageEnglish (US)
Pages (from-to)81-85
Number of pages5
JournalBiochemical Journal
Volume283
Issue number1
DOIs
StatePublished - Jan 1 1992

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Chylomicrons
Apolipoproteins
Poloxamer
Triglycerides
Apolipoprotein B-48
Apolipoprotein A-I
Apolipoproteins B
Jejunum
Messenger RNA
Apolipoproteins A
Intestinal Secretions
Esterification
Nutrition
Ileum
Surface-Active Agents
Small Intestine
Diet

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Effect of intestinal chylomicron secretory blockade on apolipoprotein synthesis in the newborn piglet. / Black, Dennis.

In: Biochemical Journal, Vol. 283, No. 1, 01.01.1992, p. 81-85.

Research output: Contribution to journalArticle

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