Effect of methoxychlor on antioxidant system of goat epididymal sperm in vitro

B. Gangadharan, M. Arul Murugan, P. P. Mathur

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Abstract

Aim: To evaluate the effect of methoxychlor on the antioxidant system of goat epididymal sperm. Methods: Epididymis of adult goat was obtained from local slaughter houses and sperm were collected by chopping the epididymis in modified Ringer's phosphate solution (RPS). After several washings, the sperm samples were dispersed in RPS and incubated with methoxychlor (1 μmol/L, 10 μmol/L and 100 μmol/L) and methoxychlor + vitamin C (100 μmol/L each) for 3 h at 32°C. After incubation, the sperm motility and viability were assessed. An aliquot of sperm sample was homogenized, centrifuged and used for the assay of superoxide dismutase, glutathione peroxidase, glutathione reductase and lipid peroxidation. Results: In methoxychlor-incubated sperm and in sperm co-incubated with methoxychlor and vitamin C, the sperm motility and viability showed no significant changes as compared to the corresponding controls. In methoxychlor-incubated sperm the activity of superoxide dismutase, glutathione reductase and glutathione peroxidase were decreased while lipid peroxidation was increased in a dose-dependent manner. Co-incubation of sperm with methoxychlor and vitamin C showed no changes in the activity of superoxide dismutase, glutathione reductase and glutathione peroxidase and in the level of lipid peroxidation. Conclusion: Methoxychlor induced oxidative stress in epididymal sperm of goats by decreasing the levels of antioxidant enzymes. Co-incubation of sperm with methoxychlor and vitamin C, a natural antioxidant, reversed the effect of methoxychlor.

Original languageEnglish (US)
Pages (from-to)285-288
Number of pages4
JournalAsian Journal of Andrology
Volume3
Issue number4
StatePublished - Dec 1 2001

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Methoxychlor
Goats
Spermatozoa
Antioxidants
Ascorbic Acid
Glutathione Reductase
Glutathione Peroxidase
Lipid Peroxidation
Superoxide Dismutase
Sperm Motility
Epididymis
Phosphates
In Vitro Techniques
Abattoirs
Oxidative Stress

All Science Journal Classification (ASJC) codes

  • Urology

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Effect of methoxychlor on antioxidant system of goat epididymal sperm in vitro. / Gangadharan, B.; Arul Murugan, M.; Mathur, P. P.

In: Asian Journal of Andrology, Vol. 3, No. 4, 01.12.2001, p. 285-288.

Research output: Contribution to journalArticle

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abstract = "Aim: To evaluate the effect of methoxychlor on the antioxidant system of goat epididymal sperm. Methods: Epididymis of adult goat was obtained from local slaughter houses and sperm were collected by chopping the epididymis in modified Ringer's phosphate solution (RPS). After several washings, the sperm samples were dispersed in RPS and incubated with methoxychlor (1 μmol/L, 10 μmol/L and 100 μmol/L) and methoxychlor + vitamin C (100 μmol/L each) for 3 h at 32°C. After incubation, the sperm motility and viability were assessed. An aliquot of sperm sample was homogenized, centrifuged and used for the assay of superoxide dismutase, glutathione peroxidase, glutathione reductase and lipid peroxidation. Results: In methoxychlor-incubated sperm and in sperm co-incubated with methoxychlor and vitamin C, the sperm motility and viability showed no significant changes as compared to the corresponding controls. In methoxychlor-incubated sperm the activity of superoxide dismutase, glutathione reductase and glutathione peroxidase were decreased while lipid peroxidation was increased in a dose-dependent manner. Co-incubation of sperm with methoxychlor and vitamin C showed no changes in the activity of superoxide dismutase, glutathione reductase and glutathione peroxidase and in the level of lipid peroxidation. Conclusion: Methoxychlor induced oxidative stress in epididymal sperm of goats by decreasing the levels of antioxidant enzymes. Co-incubation of sperm with methoxychlor and vitamin C, a natural antioxidant, reversed the effect of methoxychlor.",
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N2 - Aim: To evaluate the effect of methoxychlor on the antioxidant system of goat epididymal sperm. Methods: Epididymis of adult goat was obtained from local slaughter houses and sperm were collected by chopping the epididymis in modified Ringer's phosphate solution (RPS). After several washings, the sperm samples were dispersed in RPS and incubated with methoxychlor (1 μmol/L, 10 μmol/L and 100 μmol/L) and methoxychlor + vitamin C (100 μmol/L each) for 3 h at 32°C. After incubation, the sperm motility and viability were assessed. An aliquot of sperm sample was homogenized, centrifuged and used for the assay of superoxide dismutase, glutathione peroxidase, glutathione reductase and lipid peroxidation. Results: In methoxychlor-incubated sperm and in sperm co-incubated with methoxychlor and vitamin C, the sperm motility and viability showed no significant changes as compared to the corresponding controls. In methoxychlor-incubated sperm the activity of superoxide dismutase, glutathione reductase and glutathione peroxidase were decreased while lipid peroxidation was increased in a dose-dependent manner. Co-incubation of sperm with methoxychlor and vitamin C showed no changes in the activity of superoxide dismutase, glutathione reductase and glutathione peroxidase and in the level of lipid peroxidation. Conclusion: Methoxychlor induced oxidative stress in epididymal sperm of goats by decreasing the levels of antioxidant enzymes. Co-incubation of sperm with methoxychlor and vitamin C, a natural antioxidant, reversed the effect of methoxychlor.

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