Effect of nandrolone decanoate administration on recovery from bupivacaine-induced muscle injury

James P. White, Kristen A. Baltgalvis, Shuichi Sato, L. Britt Wilson, James Carson

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Although testosterone administration elicits well-documented anabolic effects on skeletal muscle mass, the enhancement of muscle regeneration after injury has not been widely examined. The purpose of this study was to determine whether anabolic steroid administration improves skeletal muscle regeneration from bupivacaine-induced injury. Male C57BL/6 mice were castrated 2 wk before muscle injury induced by an intramuscular bupivacaine injection into the tibialis anterior (TA) muscle. Control mice received an intramuscular PBS injection. Anabolic steroid [nandrolone decanoate (ND), 6 mg/kg] or sesame seed oil was administered at the time of initial injury and continued every 7 days for the study's duration. Mice were randomly assigned to one of four treatment groups for 5, 14, or 42 days of recovery, as follows: 1) control (uninjured); 2) ND only (uninjured + ND); 3) bupivacaine only (injured); or 4) bupivacaine + ND (injured + ND). TA morphology, protein, and gene expression were analyzed at 14 and 42 days after injury; protein expression was analyzed at 5 days after injury. After 14 days of recovery, the injury and injury + ND treatments induced small-diameter myofiber incidence and also decreased mean myofiber area. The increase in small-myofiber incidence was 65% greater in injury + ND muscle compared with injury alone. At 14 days, injury + ND induced a fivefold increase in muscle IGF-I mRNA expression, which was greater than injury alone. Muscle Akt activity and glycogen synthetase kinase-3β activity were also induced by injury + ND at 14 days of recovery, but not by injury alone. ND had a main effect for increasing muscle MyoD and cyclin D1 mRNA expression at 14 days. After 42 days of recovery, injury + ND increased large-diameter myofiber incidence compared with injury only. Nandrolone decanoate (ND) administration can enhance castrated mouse muscle regeneration during the recovery from bupivacaine-induced injury.

Original languageEnglish (US)
Pages (from-to)1420-1430
Number of pages11
JournalJournal of applied physiology
Volume107
Issue number5
DOIs
StatePublished - Nov 1 2009

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Bupivacaine
Muscles
Wounds and Injuries
Testosterone Congeners
Regeneration
nandrolone decanoate
Intramuscular Injections
Incidence
Skeletal Muscle
Sesame Oil
Anabolic Agents
Glycogen Synthase
Messenger RNA
Cyclin D1
Insulin-Like Growth Factor I
Inbred C57BL Mouse

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

Effect of nandrolone decanoate administration on recovery from bupivacaine-induced muscle injury. / White, James P.; Baltgalvis, Kristen A.; Sato, Shuichi; Britt Wilson, L.; Carson, James.

In: Journal of applied physiology, Vol. 107, No. 5, 01.11.2009, p. 1420-1430.

Research output: Contribution to journalArticle

White, James P. ; Baltgalvis, Kristen A. ; Sato, Shuichi ; Britt Wilson, L. ; Carson, James. / Effect of nandrolone decanoate administration on recovery from bupivacaine-induced muscle injury. In: Journal of applied physiology. 2009 ; Vol. 107, No. 5. pp. 1420-1430.
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