Effect of pentoxifylline on nitrogen balance and 3-methylhistidine excretion in parenterally fed endotoxemic rats

Roland Dickerson, Jay F. Mouser, J. Travis Methvin, David A. Kuhl, Emily B. Hak, Rex Brown, Lawrence J. Hak

Research output: Contribution to journalArticle

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Abstract

Pentoxifylline interrupts early gene activation for tumor necrosis factor, interleukin-1, and interleukin-6 production and improves survival from experimental sepsis. These effects can alter nitrogen loss during critical illness. To determine the dose-dependent influence of pentoxifylline on nitrogen loss, 44 male Sprague-Dawley rats (220 to 265 g) were randomized to receive parenteral nutrition only (PN), PN plus continuous infusion of Escherichia coli 026:B6 lipopolysaccharide (LPS) at 9 mg · kg-1 · d-1, or PN plus LPS plus a continuous infusion of pentoxifylline at either 25 (PEN25) or 100 mg · kg-1 · d-1 (PEN100) for 48 h. Before randomization, all animals underwent intravenous cannulation and 40 h of PN adaptation. All animals received isocaloric, isonitrogenous PN (160 kcal · kg-1 · d-1 and 1.0 gN · kg-1 · d-1) and were kept nil per os except for water ad libitum. Administration of LPS significantly worsened nitrogen balance for all three groups compared with PN control; however, pentoxifylline only modestly improved nitrogen balance compared with LPS (206 ± 255, -497 ± 331, -332 ± 329, and -310 ± 383 mg/48hr for the PN, LPS, PEN25, and PEN100 groups, respectively; P < 0.001). Pentoxifylline did not significantly change 3-methylhistidine urinary excretion compared with LPS (573 ± 180, 705 ± 156, 780 ± 326, and 683 ± 266 μg/48 h for the PN, LPS, PEN25, and PEN100 groups, respectively, P not significant). Pentoxifylline, given in therapeutic doses after an endotoxin challenge, modestly, but not significantly, improved nitrogen balance. Urinary 3-methylhistidine excretion was not influenced by pentoxifylline. A dose-dependent effect by pentoxifylline on these markers was not evident.

Original languageEnglish (US)
Pages (from-to)623-627
Number of pages5
JournalNutrition
Volume17
Issue number7-8
DOIs
StatePublished - Jul 24 2001

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Pentoxifylline
Parenteral Nutrition
Nitrogen
Lipopolysaccharides
3-methylhistidine
Random Allocation
Interleukin-1
Critical Illness
Endotoxins
Catheterization
Transcriptional Activation
Sprague Dawley Rats
Interleukin-6
Sepsis
Tumor Necrosis Factor-alpha
Escherichia coli

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Effect of pentoxifylline on nitrogen balance and 3-methylhistidine excretion in parenterally fed endotoxemic rats. / Dickerson, Roland; Mouser, Jay F.; Methvin, J. Travis; Kuhl, David A.; Hak, Emily B.; Brown, Rex; Hak, Lawrence J.

In: Nutrition, Vol. 17, No. 7-8, 24.07.2001, p. 623-627.

Research output: Contribution to journalArticle

Dickerson, Roland ; Mouser, Jay F. ; Methvin, J. Travis ; Kuhl, David A. ; Hak, Emily B. ; Brown, Rex ; Hak, Lawrence J. / Effect of pentoxifylline on nitrogen balance and 3-methylhistidine excretion in parenterally fed endotoxemic rats. In: Nutrition. 2001 ; Vol. 17, No. 7-8. pp. 623-627.
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