Effect of platelet-activating factor on basal and insulin-mediated system A amino acid transport in rat soleus muscle

J. S. Boruff, Michael Karlstad

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We investigated the effect of platelet-activating factor (PAF) on basal and insulin-stimulated sodium-dependent neutral amino acid transport by system A in rat soleus muscle. Fasted male Sprague-Dawley rats (75-100 g) were given an intravenous injection of saline or PAF (4 μg/kg) and killed 1 hr later. Isolated soleus muscles were incubated in media containing 1- 14C-α-aminoisobutyric acid (AIB; system A amino acid analog), 3H-inulin, and 0 (basal) or 100 mU/ml insulin. Na+-free media were assessed by Na+- independent AIB uptake. The rate of AIB uptake by muscle was corrected for uptake into extracellular (inulin) space. Basal cellular AIB uptake by soleus muscles from PAF-treated rats was 30.2% lower than in controls. Insulin increased the absolute rate of cellular AIB uptake above basal levels to the same extent in muscles of control and PAF-treated rats, although the rate of maximal insulin-stimulated AIB uptake was 20% lower in muscles of PAF- treated rats. Na+-independent AIB uptake (measured in Na+-free media) was the same in muscles of control and PAF-treated rats. The fact that AIB uptake in Na+-free media was the same in muscles of control and PAF-treated rats indicates that the reduction in basal and insulin-stimulated AIB uptake in muscles of PAF-treated rats was due to an alteration of system A Na+- dependent amino acid transport.

Original languageEnglish (US)
Pages (from-to)75-80
Number of pages6
JournalCirculatory Shock
Volume40
Issue number1
StatePublished - 1993
Externally publishedYes

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Amino Acid Transport Systems
Platelet Activating Factor
Skeletal Muscle
Insulin
Muscles
Inulin
Amino Acid Transport System A
Neutral Amino Acid Transport Systems
Aminoisobutyric Acids
Platelet Factor 4
Amino Acids
Extracellular Space
Intravenous Injections
Sprague Dawley Rats

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

Cite this

Effect of platelet-activating factor on basal and insulin-mediated system A amino acid transport in rat soleus muscle. / Boruff, J. S.; Karlstad, Michael.

In: Circulatory Shock, Vol. 40, No. 1, 1993, p. 75-80.

Research output: Contribution to journalArticle

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