Effect of Probiotic dahi (CURD) on cytogenetical profile during 1,2-dimethylhydrazine induced colon carcinogenesis in rats

Raj Kumar, P. R. Sinha, B. R. Yadav, Arvind, Nikhlesh Singh, Rakesh Tonk

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In human, colon cancer is the major health problem worldwide. It arises by a well-defined series of histopathological changes (adenoma-carcinoma sequence), which are paralleled by mutations and deletions of tumor suppressor genes leading to chromosomal aberrations. Chromosomal aberrations are considered to be good somatic biomarkers of colon cancer and occur with the greatest frequency in lymphocytes. The present work was undertaken with objective to study the protective effect of probiotic dahi on generation of chromosomal aberrations (numerical and structural) in lymphocytes of 1, 2-dimethylhydrazine induced colon carcinogenesis in rats. The rats were divided into four groups 1) Normal diet control group; 2) DMH control; 3) Normal dahi (ND) group; 4) Probiotic dahi (PD) group. Rats were injected with DMH and sacrificed at 50th week of experiment, metaphasic chromosomes were prepared from blood lymphocytes and incidences of chromosomal aberrations were studied. In normal diet group, DMH control group, ND group and in PD group incidence of chromosomal aberrations were 2.0, 15.33, 12.0 and 4.66% respectively. Administration of probiotic dahi to rats significantly decreased the incidence of chromosomal aberrations in lymphocytes as compared to DMH control rats and rats fed with normal dahi (P< 0.05). The findings of present study suggest that probiotic dahi exerts its antigenotoxic effects by preventing the chromosomal aberrations.

Original languageEnglish (US)
Pages (from-to)45-50
Number of pages6
JournalInternational Journal of Probiotics and Prebiotics
Volume4
Issue number1
StatePublished - Feb 1 2009

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1,2-dimethylhydrazine
1,2-Dimethylhydrazine
chromosome aberrations
Probiotics
Chromosome Aberrations
carcinogenesis
probiotics
colon
Dimenhydrinate
Colon
Carcinogenesis
rats
lymphocytes
Lymphocytes
colorectal neoplasms
incidence
Colonic Neoplasms
Incidence
Diet
Control Groups

All Science Journal Classification (ASJC) codes

  • Food Animals
  • Applied Microbiology and Biotechnology
  • Nutrition and Dietetics
  • Public Health, Environmental and Occupational Health

Cite this

Effect of Probiotic dahi (CURD) on cytogenetical profile during 1,2-dimethylhydrazine induced colon carcinogenesis in rats. / Kumar, Raj; Sinha, P. R.; Yadav, B. R.; Arvind, ; Singh, Nikhlesh; Tonk, Rakesh.

In: International Journal of Probiotics and Prebiotics, Vol. 4, No. 1, 01.02.2009, p. 45-50.

Research output: Contribution to journalArticle

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