Effect of Thimerosal in Leukemia, in Leukemic Cell Lines, and on Normal Hematopoiesis

Timothy Panella, Andrew T. Huang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Anti-thymocyte globulin (ATG), a horse antiserum to human thymus tissue, has been shown to induce granulocytic differentiation of the HL-60 human leukemia cell line. In this paper we describe the effect of ATG on leukemic blasts and its effect on other human leukemia cell lines in vitro. The in vitro differentiation effect of ATG was observed in blasts from two patients with leukemia and the human leukemia cell line K562. The differentiation effect of ATG was attributable to its preservative, thimerosal, separable from ATG by high pressure liquid chromatography or dialysis. Subsequent studies with thimerosal alone showed it to induce differentiation in leukemic blasts from three patients and the human leukemia cell lines U937, K562, and KG-1. The differentiation effect of thimerosal is blocked by a sulfhydryl-protective agent, dithiothreitol, suggesting that the mechanism of differentiation may be mediated via a sulfhydryl group-dependent process.

Original languageEnglish (US)
Pages (from-to)4429-4435
Number of pages7
JournalCancer Research
Volume50
Issue number14
StatePublished - Jul 15 1990
Externally publishedYes

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Thimerosal
Antilymphocyte Serum
Hematopoiesis
Leukemia
Cell Line
Protective Agents
Group Processes
Dithiothreitol
Thymus Gland
Horses
Immune Sera
Dialysis
High Pressure Liquid Chromatography

All Science Journal Classification (ASJC) codes

  • Cancer Research
  • Oncology

Cite this

Effect of Thimerosal in Leukemia, in Leukemic Cell Lines, and on Normal Hematopoiesis. / Panella, Timothy; Huang, Andrew T.

In: Cancer Research, Vol. 50, No. 14, 15.07.1990, p. 4429-4435.

Research output: Contribution to journalArticle

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