Effect of ursodeoxycholic acid on cholesterol absorption and metabolism in humans

Laura A. Woollett, Donna D. Buckley, Lihang Yao, Peter J.H. Jones, Norman A. Granholm, Elizabeth Tolley, James E. Heubi

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Qualitative and quantitative changes in intraluminal bile acid composition may alter cholesterol absorption and synthesis and LDL receptor expression. In a randomized crossover design outpatient study, 12 adults aged 24-36 years took 15 mg/kg/day ursodeoxycholic acid (UDCA) or no bile acid supplement (control) for 20 days while being fed a controlled diet (AHA Step II). A liquid meal of defined composition was then given and luminal samples collected. Cholesterol absorption and cholesterol fractional synthetic rate (FSR) were assessed by stable isotopic methods. With UDCA treatment, bile was enriched significantly (P < 0.0001) to 40.6 ± 2.6% (mean ± SEM) compared with 2.2 ± 2.6% for controls. Regardless, plasma total, HDL, and LDL cholesterol were unchanged with UDCA treatment. Intraluminal cholesterol solubilized in the aqueous phase during the entire collection was decreased (P = 0.012) in UDCA-treated subjects (101.0 ± 7.2 mg/ml/120 min) compared with controls (132.5 ± 7.2 mg/ml/120 min.). Percent micellar cholesterol was increased in UDCA-treated versus controls after meal ingestion. No changes were found in cholesterol absorption, FSR, or LDL receptor mRNA with UDCA treatment compared with controls. Thus, despite marked enrichment in luminal bile with UDCA and decreased cholesterol solubilization, no differences in cholesterol absorption or metabolism are found when diet and genetic differences in absorption are carefully controlled.

Original languageEnglish (US)
Pages (from-to)935-942
Number of pages8
JournalJournal of lipid research
Volume44
Issue number5
DOIs
StatePublished - May 1 2003

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Ursodeoxycholic Acid
Metabolism
Cholesterol
LDL Receptors
Nutrition
Bile Acids and Salts
Bile
Meals
Artificial Receptors
Diet
Chemical analysis
LDL Cholesterol
Cross-Over Studies
HDL Cholesterol
Outpatients
Therapeutics
Eating
Plasmas
Messenger RNA
Scanning electron microscopy

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Endocrinology
  • Cell Biology

Cite this

Woollett, L. A., Buckley, D. D., Yao, L., Jones, P. J. H., Granholm, N. A., Tolley, E., & Heubi, J. E. (2003). Effect of ursodeoxycholic acid on cholesterol absorption and metabolism in humans. Journal of lipid research, 44(5), 935-942. https://doi.org/10.1194/jlr.M200478-JLR200

Effect of ursodeoxycholic acid on cholesterol absorption and metabolism in humans. / Woollett, Laura A.; Buckley, Donna D.; Yao, Lihang; Jones, Peter J.H.; Granholm, Norman A.; Tolley, Elizabeth; Heubi, James E.

In: Journal of lipid research, Vol. 44, No. 5, 01.05.2003, p. 935-942.

Research output: Contribution to journalArticle

Woollett, LA, Buckley, DD, Yao, L, Jones, PJH, Granholm, NA, Tolley, E & Heubi, JE 2003, 'Effect of ursodeoxycholic acid on cholesterol absorption and metabolism in humans', Journal of lipid research, vol. 44, no. 5, pp. 935-942. https://doi.org/10.1194/jlr.M200478-JLR200
Woollett, Laura A. ; Buckley, Donna D. ; Yao, Lihang ; Jones, Peter J.H. ; Granholm, Norman A. ; Tolley, Elizabeth ; Heubi, James E. / Effect of ursodeoxycholic acid on cholesterol absorption and metabolism in humans. In: Journal of lipid research. 2003 ; Vol. 44, No. 5. pp. 935-942.
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