Effectiveness of curcumin against enterococcus faecalis biofilm

Prasanna Neelakantan, Chandana Subbarao, Subash Sharma, Chandragiri Venkata Subbarao, Franklin Garcia-Godoy, James L. Gutmann

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Objectives. To evaluate the antimicrobial efficacy of curcumin against Enterococcus faecalis biolm formed on tooth substrate in vitro. Sodium hypochlorite (NaOCl) and chlorhexidine (CHX) served as standards for comparison. Materials and methods. Biofilms of E.faecalis were formed on instrumented, extracted human teeth (n = 96). At the end of the 2nd day, 2nd and 8th weeks, specimens were treated for 30 min with one of the test solutions or saline (control) and the surviving colony-forming units (CFU/mL) was recorded. Results were analyzed by Kruskal-Wallis test and Dunnet test for pair-wise comparison with Bonferroni correction (p = 0.05). Results. Only NaOCl showed complete eradication of bacteria at all time periods. In the 2-day and 2nd week biofilms, curcumin and NaOCl showed complete inhibition, which was significantly lower than the CFU recovered in the CHX and saline groups (p < 0.05). In 8 week biofilms, samples treated with curcumin showed 553 ± 137.6 CFU/mL, which was significantly higher than NaOCl (0 CFU/mL), but significantly lower than CHX (2551 ± 129.8) and saline control (1.42 × 1011 ± 2.12 × 1010; p < 0.05). Conclusions. Sodium hypochlorite (3%) showed maximum antibacterial activity against E.faecalis biolm formed on the tooth substrate, followed by curcumin and CHX. Considering the potential for undesirable properties of NaOCl, the use of herbal alternatives in endodontics might prove to be advantageous.

Original languageEnglish (US)
Pages (from-to)1453-1457
Number of pages5
JournalActa Odontologica Scandinavica
Volume71
Issue number6
DOIs
StatePublished - Nov 1 2013

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Curcumin
Chlorhexidine
Enterococcus faecalis
Biofilms
Sodium Hypochlorite
Tooth
Endodontics
Sodium Chloride
Stem Cells
Bacteria

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Neelakantan, P., Subbarao, C., Sharma, S., Subbarao, C. V., Garcia-Godoy, F., & Gutmann, J. L. (2013). Effectiveness of curcumin against enterococcus faecalis biofilm. Acta Odontologica Scandinavica, 71(6), 1453-1457. https://doi.org/10.3109/00016357.2013.769627

Effectiveness of curcumin against enterococcus faecalis biofilm. / Neelakantan, Prasanna; Subbarao, Chandana; Sharma, Subash; Subbarao, Chandragiri Venkata; Garcia-Godoy, Franklin; Gutmann, James L.

In: Acta Odontologica Scandinavica, Vol. 71, No. 6, 01.11.2013, p. 1453-1457.

Research output: Contribution to journalArticle

Neelakantan, P, Subbarao, C, Sharma, S, Subbarao, CV, Garcia-Godoy, F & Gutmann, JL 2013, 'Effectiveness of curcumin against enterococcus faecalis biofilm', Acta Odontologica Scandinavica, vol. 71, no. 6, pp. 1453-1457. https://doi.org/10.3109/00016357.2013.769627
Neelakantan, Prasanna ; Subbarao, Chandana ; Sharma, Subash ; Subbarao, Chandragiri Venkata ; Garcia-Godoy, Franklin ; Gutmann, James L. / Effectiveness of curcumin against enterococcus faecalis biofilm. In: Acta Odontologica Scandinavica. 2013 ; Vol. 71, No. 6. pp. 1453-1457.
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