Effects of 4-aminopyridine (4-AP) on rat neostriatal neurons in an in vitro slice preparation

T. Kita, Hitoshi Kita, S. T. Kitai

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Effects of 4-aminopyridine (4-AP) on the rat neostriatal neuron were studied using the in vitro slice preparation. The intracellularly recorded neurons had resting membrane potentials of more than 50 mV and were capable of generating acid potentials with the amplitude greater than 60 mV. Application of 4-AP in the superfusing media depolarizes the cell membrane and increases its input resistance. Local electrical stimulation induces excitatory postsynaptic potentials (EPSPs) overlapping with inhibitory postsynaptic potentials (IPSPs) in these neurons. 4-AP application enhances the amplitude and duration of the postsynaptic potentials. With application of higher concentration of 4-AP, local stimulation induces a second EPSP and a bicuculline sensitive long duration depolarization. These results indicate that 4-AP clearly has effects on local stimulation-induced postsynaptic responses of neostriatal neurons. Possible mechanisms underlying the 4-AP actions on neurotransmission in the neostriatal slice are discussed.

Original languageEnglish (US)
Pages (from-to)10-18
Number of pages9
JournalBrain Research
Volume361
Issue number1-2
DOIs
StatePublished - Dec 30 1985

Fingerprint

4-Aminopyridine
Neurons
Excitatory Postsynaptic Potentials
Inhibitory Postsynaptic Potentials
Synaptic Potentials
Bicuculline
Synaptic Transmission
Membrane Potentials
Electric Stimulation
In Vitro Techniques
Cell Membrane
Acids

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Effects of 4-aminopyridine (4-AP) on rat neostriatal neurons in an in vitro slice preparation. / Kita, T.; Kita, Hitoshi; Kitai, S. T.

In: Brain Research, Vol. 361, No. 1-2, 30.12.1985, p. 10-18.

Research output: Contribution to journalArticle

@article{316e4071791846cb92587a98adea1eb8,
title = "Effects of 4-aminopyridine (4-AP) on rat neostriatal neurons in an in vitro slice preparation",
abstract = "Effects of 4-aminopyridine (4-AP) on the rat neostriatal neuron were studied using the in vitro slice preparation. The intracellularly recorded neurons had resting membrane potentials of more than 50 mV and were capable of generating acid potentials with the amplitude greater than 60 mV. Application of 4-AP in the superfusing media depolarizes the cell membrane and increases its input resistance. Local electrical stimulation induces excitatory postsynaptic potentials (EPSPs) overlapping with inhibitory postsynaptic potentials (IPSPs) in these neurons. 4-AP application enhances the amplitude and duration of the postsynaptic potentials. With application of higher concentration of 4-AP, local stimulation induces a second EPSP and a bicuculline sensitive long duration depolarization. These results indicate that 4-AP clearly has effects on local stimulation-induced postsynaptic responses of neostriatal neurons. Possible mechanisms underlying the 4-AP actions on neurotransmission in the neostriatal slice are discussed.",
author = "T. Kita and Hitoshi Kita and Kitai, {S. T.}",
year = "1985",
month = "12",
day = "30",
doi = "10.1016/0006-8993(85)91269-7",
language = "English (US)",
volume = "361",
pages = "10--18",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Effects of 4-aminopyridine (4-AP) on rat neostriatal neurons in an in vitro slice preparation

AU - Kita, T.

AU - Kita, Hitoshi

AU - Kitai, S. T.

PY - 1985/12/30

Y1 - 1985/12/30

N2 - Effects of 4-aminopyridine (4-AP) on the rat neostriatal neuron were studied using the in vitro slice preparation. The intracellularly recorded neurons had resting membrane potentials of more than 50 mV and were capable of generating acid potentials with the amplitude greater than 60 mV. Application of 4-AP in the superfusing media depolarizes the cell membrane and increases its input resistance. Local electrical stimulation induces excitatory postsynaptic potentials (EPSPs) overlapping with inhibitory postsynaptic potentials (IPSPs) in these neurons. 4-AP application enhances the amplitude and duration of the postsynaptic potentials. With application of higher concentration of 4-AP, local stimulation induces a second EPSP and a bicuculline sensitive long duration depolarization. These results indicate that 4-AP clearly has effects on local stimulation-induced postsynaptic responses of neostriatal neurons. Possible mechanisms underlying the 4-AP actions on neurotransmission in the neostriatal slice are discussed.

AB - Effects of 4-aminopyridine (4-AP) on the rat neostriatal neuron were studied using the in vitro slice preparation. The intracellularly recorded neurons had resting membrane potentials of more than 50 mV and were capable of generating acid potentials with the amplitude greater than 60 mV. Application of 4-AP in the superfusing media depolarizes the cell membrane and increases its input resistance. Local electrical stimulation induces excitatory postsynaptic potentials (EPSPs) overlapping with inhibitory postsynaptic potentials (IPSPs) in these neurons. 4-AP application enhances the amplitude and duration of the postsynaptic potentials. With application of higher concentration of 4-AP, local stimulation induces a second EPSP and a bicuculline sensitive long duration depolarization. These results indicate that 4-AP clearly has effects on local stimulation-induced postsynaptic responses of neostriatal neurons. Possible mechanisms underlying the 4-AP actions on neurotransmission in the neostriatal slice are discussed.

UR - http://www.scopus.com/inward/record.url?scp=0022352733&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022352733&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(85)91269-7

DO - 10.1016/0006-8993(85)91269-7

M3 - Article

VL - 361

SP - 10

EP - 18

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1-2

ER -