Effects of adenosine and protein kinase C stimulation on mechanical properties of rat cardiac myocytes

J. W. Lester, K. F. Gannaway, R. A. Reardon, L. D. Koon, Polly Hofmann

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Exposure of the heart to adenosine decreases heart rate and left ventricular developed pressure. However, little is known regarding the influence of adenosine on mechanical properties of isolated ventricular myocytes and the intracellular mechanism(s) by which adenosine acts. Therefore, in the present study we compared the effects of the adenosine receptor agonist R-phenylisopropyladenosine (R-PIA) and protein kinase C (PKC) activator dioctanoylglycerol (DOG) on Ca 2+ sensitivity of tension, maximum isometric tension, and velocity of unloaded shortening (V(max)) in enzymatically isolated, drug-treated, and subsequently skinned ventricular myocytes. Neither R-PIA (100 μM) nor DOG (50 μM) affected Ca 2+ sensitivity of tension or maximum isometric tension compared with controls. However, both R-PIA and DOG treatment caused ~25% decrease in V(max) during maximum activation compared with controls. This suggests adenosine and PKC decrease actin-myosin interaction through an alteration of myofilament proteins. The observed similarity of response after R-PIA and DOG treatment is consistent with the hypothesis that effects of adenosine are mediated by activation of the PKC pathway in isolated ventricular myocytes.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume271
Issue number5 40-5
StatePublished - Nov 1 1996

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Phenylisopropyladenosine
Adenosine Kinase
Cardiac Myocytes
Adenosine
Protein Kinase C
Muscle Cells
Purinergic P1 Receptor Agonists
Myofibrils
Ventricular Pressure
Myosins
Actins
Heart Rate
Pharmaceutical Preparations
Proteins

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Effects of adenosine and protein kinase C stimulation on mechanical properties of rat cardiac myocytes. / Lester, J. W.; Gannaway, K. F.; Reardon, R. A.; Koon, L. D.; Hofmann, Polly.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 271, No. 5 40-5, 01.11.1996.

Research output: Contribution to journalArticle

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