Effects of allopurinol pretreatment with pulmonary flush on lung preservation

M. Aiba, Y. Yokoyama, T. R. Snow, D. Novitzky, Peter Mckeown

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

This study was designed to test whether use of allopurinol could improve lung preservation after 6 hours of cold storage. Thirty-two rabbits were divided into four groups (n=8 each group): (1) the control group received no flush or storage, (2) the EC group received Euro-Collins (EC) solution for both flush and storage, (3) the Allo-F group received Euro-Collins solution with allopurinol (1mmol/L) for both flush and storage, and (4) the Allo-R group received Euro-Collins solution to which allopurinol (1mmol/L) was added only to the reperfused blood. For groups 2 through 4, the lungs were flushed (40ml/kg) in situ, excised, and then stored at 4°C. After storage, the lungs were reperfused for 1 hour with an in vitro blood-perfused ventilated model. Lung function was measured during reperfusion with mean pulmonary arterial pressure, end-inspiratory airway pressure, and blood gas data. The lung wet/dry weight ratio was used to measure lung edema. The lungs in the EC group had a significant increase in mean pulmonary arterial pressure, airway pressure, and wet/dry weight ratio when compared with the control group. The mean pulmonary arterial pressure in either of the groups receiving allopurinol was consistently lower than that in the EC group. The airway pressure in the Allo-R group also significantly decreased compared with the EC group. The wet/dry weight ratios in both of the allopurinol-treated groups were significantly lower than in the EC group (6.02±0.31 in the Allo-F group and 5.60±0.22 in the Allo-R group versus 7.92±0.67 in the EC group, p<0.05). The blood gases, PO2 and PCO2, did not differ among the four groups at any point during the reperfusion. We conclude that allopurinol added to the pulmonary flush solution or to the perfusate provides better lung preservation after 6-hour cold storage.

Original languageEnglish (US)
Pages (from-to)1025-1030
Number of pages6
JournalJournal of Heart and Lung Transplantation
Volume11
Issue number6
StatePublished - Dec 1 1992
Externally publishedYes

Fingerprint

Allopurinol
Lung
Arterial Pressure
Weights and Measures
Reperfusion
Gases
Pressure
Control Groups
Pulmonary Edema
Edema
Rabbits
Blood Pressure

All Science Journal Classification (ASJC) codes

  • Surgery
  • Pulmonary and Respiratory Medicine
  • Cardiology and Cardiovascular Medicine
  • Transplantation

Cite this

Effects of allopurinol pretreatment with pulmonary flush on lung preservation. / Aiba, M.; Yokoyama, Y.; Snow, T. R.; Novitzky, D.; Mckeown, Peter.

In: Journal of Heart and Lung Transplantation, Vol. 11, No. 6, 01.12.1992, p. 1025-1030.

Research output: Contribution to journalArticle

Aiba, M. ; Yokoyama, Y. ; Snow, T. R. ; Novitzky, D. ; Mckeown, Peter. / Effects of allopurinol pretreatment with pulmonary flush on lung preservation. In: Journal of Heart and Lung Transplantation. 1992 ; Vol. 11, No. 6. pp. 1025-1030.
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