Effects of atmospheric non-thermal argon/oxygen plasma on biofilm viability and hydrophobicity of oral bacteria

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Abstract

Purpose: To evaluate the bactericidal effects of atmospheric non-thermal argon/oxygen plasma on in vitro oral biofilms constructed from S. mutans and/or S. sanguinis, and the influence of the plasma on the virulence properties of A. oris. Methods: In vitro oral biofilms were constructed in the wells of 48-well plates from S. mutans and/or S. sanguinis. The wells containing constructed biofilms and various amounts of phosphate-buffered saline (PBS) were treated with nonthermal argon/oxygen plasma brush for 2 minutes. The methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay and Live/Dead assay were used to evaluate the viability of biofilms in those wells after the plasma treatments. Meanwhile, A. oris suspensions were treated with the plasma and then evaluated for their virulence properties by measuring the hydrophobicity and co-aggregation capability of treated A. oris. Results: The MTT assay showed that exposure to nonthermal plasma for 2 minutes significantly reduced the viability of bacteria in both single-species and two-species biofilms of S. mutans and S. sanguinis with the reductions of up to 99%. Meanwhile, plasma treatment also altered the hydrophobicity of A. oris, and reduced their capability to co-aggregate with S. sanguinis.

Original languageEnglish (US)
Pages (from-to)52-56
Number of pages5
JournalAmerican journal of dentistry
Volume30
Issue number1
StatePublished - Feb 1 2017

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Argon
Biofilms
Hydrophobic and Hydrophilic Interactions
Oxygen
Bacteria
Bromides
Virulence
Microbial Viability
Suspensions
Phosphates

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

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title = "Effects of atmospheric non-thermal argon/oxygen plasma on biofilm viability and hydrophobicity of oral bacteria",
abstract = "Purpose: To evaluate the bactericidal effects of atmospheric non-thermal argon/oxygen plasma on in vitro oral biofilms constructed from S. mutans and/or S. sanguinis, and the influence of the plasma on the virulence properties of A. oris. Methods: In vitro oral biofilms were constructed in the wells of 48-well plates from S. mutans and/or S. sanguinis. The wells containing constructed biofilms and various amounts of phosphate-buffered saline (PBS) were treated with nonthermal argon/oxygen plasma brush for 2 minutes. The methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay and Live/Dead assay were used to evaluate the viability of biofilms in those wells after the plasma treatments. Meanwhile, A. oris suspensions were treated with the plasma and then evaluated for their virulence properties by measuring the hydrophobicity and co-aggregation capability of treated A. oris. Results: The MTT assay showed that exposure to nonthermal plasma for 2 minutes significantly reduced the viability of bacteria in both single-species and two-species biofilms of S. mutans and S. sanguinis with the reductions of up to 99{\%}. Meanwhile, plasma treatment also altered the hydrophobicity of A. oris, and reduced their capability to co-aggregate with S. sanguinis.",
author = "Tianshuang Liu and Linfeng Wu and Jegdish Babu and Timothy Hottel and Franklin Garcia-Godoy and Liang Hong",
year = "2017",
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T1 - Effects of atmospheric non-thermal argon/oxygen plasma on biofilm viability and hydrophobicity of oral bacteria

AU - Liu, Tianshuang

AU - Wu, Linfeng

AU - Babu, Jegdish

AU - Hottel, Timothy

AU - Garcia-Godoy, Franklin

AU - Hong, Liang

PY - 2017/2/1

Y1 - 2017/2/1

N2 - Purpose: To evaluate the bactericidal effects of atmospheric non-thermal argon/oxygen plasma on in vitro oral biofilms constructed from S. mutans and/or S. sanguinis, and the influence of the plasma on the virulence properties of A. oris. Methods: In vitro oral biofilms were constructed in the wells of 48-well plates from S. mutans and/or S. sanguinis. The wells containing constructed biofilms and various amounts of phosphate-buffered saline (PBS) were treated with nonthermal argon/oxygen plasma brush for 2 minutes. The methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay and Live/Dead assay were used to evaluate the viability of biofilms in those wells after the plasma treatments. Meanwhile, A. oris suspensions were treated with the plasma and then evaluated for their virulence properties by measuring the hydrophobicity and co-aggregation capability of treated A. oris. Results: The MTT assay showed that exposure to nonthermal plasma for 2 minutes significantly reduced the viability of bacteria in both single-species and two-species biofilms of S. mutans and S. sanguinis with the reductions of up to 99%. Meanwhile, plasma treatment also altered the hydrophobicity of A. oris, and reduced their capability to co-aggregate with S. sanguinis.

AB - Purpose: To evaluate the bactericidal effects of atmospheric non-thermal argon/oxygen plasma on in vitro oral biofilms constructed from S. mutans and/or S. sanguinis, and the influence of the plasma on the virulence properties of A. oris. Methods: In vitro oral biofilms were constructed in the wells of 48-well plates from S. mutans and/or S. sanguinis. The wells containing constructed biofilms and various amounts of phosphate-buffered saline (PBS) were treated with nonthermal argon/oxygen plasma brush for 2 minutes. The methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay and Live/Dead assay were used to evaluate the viability of biofilms in those wells after the plasma treatments. Meanwhile, A. oris suspensions were treated with the plasma and then evaluated for their virulence properties by measuring the hydrophobicity and co-aggregation capability of treated A. oris. Results: The MTT assay showed that exposure to nonthermal plasma for 2 minutes significantly reduced the viability of bacteria in both single-species and two-species biofilms of S. mutans and S. sanguinis with the reductions of up to 99%. Meanwhile, plasma treatment also altered the hydrophobicity of A. oris, and reduced their capability to co-aggregate with S. sanguinis.

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