Effects of chronic adult periodontitis and endotoxin (LPS) on gingival fibroblast plasma membrane Ca++-pump

Sidney Stein, J. L. Borke, L. A. Cummings

Research output: Contribution to journalArticle

Abstract

Gingival fibroblasts from patients with chronic adult periodontitis are known to produce cytokines in response to changing levels of bacterial lipopolysaccharide (LPS). Cytokine production is one of numerous cell processes that involve calcium dependent enzymes. It is possible that inflammation may induce changes in the amount of the Ca++ -pump protein in gingival fibroblasts which could alter Ca++-dependent activities in these cells including the production and release of cytokines. The purpose of this study was to determine if differences exist in the amount of Ca++-pump protein in the gingival fibroblasts of periodontitis patients relative to control individuals without periodontal disease. Fibroblast explants from healthy tissue and from inflamed tissue from patients with chronic adult periodontitis, grown in culture, were analyzed for quantitative differences in the amount of Ca++-pump protein. Fibroblasts from chronic adult periodontitis patients exhibited significantly lower levels of Ca++-pump protein than fibroblasts from healthy subjects (p=0.0015). However, fibroblasts from chronic adult periodontitis patients, when activated with LPS, did not exhibit significant differences in the amounts of Ca++-pump protein as compared to untreated controls (p = 0.2177). Similarly, cells from healthy subjects did not show significant reduction in Ca++-pump protein following activation with LPS (p= 0.1732). Our results suggest that plasma membrane Ca++-pump is significantly reduced in fibroblasts derived from patients with chronic periodontitis. However, factors other than LPS may be involved in the downregulation of Ca++-pump protein.

Original languageEnglish (US)
Pages (from-to)59-66
Number of pages8
JournalConnective Tissue Research
Volume40
Issue number1
DOIs
StatePublished - Jan 1 1999

Fingerprint

Chronic Periodontitis
Fibroblasts
Cell membranes
Endotoxins
Lipopolysaccharides
Cell Membrane
Pumps
Proteins
Cytokines
Healthy Volunteers
Tissue
Periodontitis
Periodontal Diseases
Chemical activation
Down-Regulation
Calcium
Inflammation
Enzymes

All Science Journal Classification (ASJC) codes

  • Rheumatology
  • Biochemistry
  • Orthopedics and Sports Medicine
  • Molecular Biology
  • Cell Biology

Cite this

Effects of chronic adult periodontitis and endotoxin (LPS) on gingival fibroblast plasma membrane Ca++-pump. / Stein, Sidney; Borke, J. L.; Cummings, L. A.

In: Connective Tissue Research, Vol. 40, No. 1, 01.01.1999, p. 59-66.

Research output: Contribution to journalArticle

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