Effects of chronic cold exposure on the endothelin system

Gin Fu Chen, Zhongjie Sun

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Cold temperatures have adverse effects on the human cardiovascular system. Endothelin (ET)-1 is a potent vasoconstrictor. We hypothesized that cold exposure increases ET-1 production and upregulates ET type A (ET A ) receptors. The aim of this study was to determine the effect of cold exposure on regulation of the ET system. Four groups of rats (6-7 rats/group) were used: three groups were exposed to moderate cold (6.7 ± 2°C) for 1, 3, and 5 wk, respectively, and the remaining group was maintained at room temperature (25°C) and served as control. Cold exposure significantly increased ET-1 levels in the heart, mesenteric arteries, renal cortex, and renal medulla. Cold exposure increased ET A receptor protein expression in the heart and renal cortex. ET type B (ET B ) receptor expression, however, was decreased significantly in the heart and renal medulla of cold-exposed rats. Cold exposure significantly increased the ratio of ET A to ET B receptors in the heart. An additional four groups of rats (3 rats/group) were used to localize changes in ET A and ET B receptors at 1, 3, and 5 wk of cold exposure. Immunohistochemical analysis showed an increase in ET A , but a decrease in ET B , receptor immunoreactivity in cardiomyocytes of cold-exposed rats. Increased ET A receptor immunoreactivity was also found in vascular smooth muscle cells of cold-exposed rats. Cold exposure increased ET A receptor immunoreactivity in tubule epithelial cells in the renal cortex but decreased ET B receptor immunoreactivity in tubule epithelial cells in the renal medulla. Therefore, cold exposure increased ET-1 production, upregulated ET A receptors, and downregulated ET B receptors.

Original languageEnglish (US)
Pages (from-to)1719-1726
Number of pages8
JournalJournal of Applied Physiology
Volume100
Issue number5
DOIs
StatePublished - May 1 2006
Externally publishedYes

Fingerprint

Endothelins
Endothelin B Receptors
Endothelin A Receptors
Endothelin-1
Kidney
Epithelial Cells
Mesenteric Arteries
Vasoconstrictor Agents
Cardiovascular System
Vascular Smooth Muscle
Cardiac Myocytes
Smooth Muscle Myocytes
Up-Regulation
Down-Regulation

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

Effects of chronic cold exposure on the endothelin system. / Chen, Gin Fu; Sun, Zhongjie.

In: Journal of Applied Physiology, Vol. 100, No. 5, 01.05.2006, p. 1719-1726.

Research output: Contribution to journalArticle

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