Effects of elevated Po2 upon tracheal explants

S. D. Barnes, C. C. Agee, R. J. Peace, Charles Leffler

Research output: Contribution to journalArticle

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Abstract

Time-dependent effects of elevated Po2 upon hamster tracheal rings in culture were examined. Ciliary activity of rings in 0.20 and 0.40 O2 remained stable for 144 h. Ciliary activity decreased 25% following 120 h exposure to 0.60 O2 and 50% within 72 h exposure to 0.95 O2. Ciliostasis occuredd by 144 h at 0.95 O2. [3H]Leucine uptake progressively decreased during 24 to 144 h exposure to 0.40, 0.60, and 0.95 O2 when compared to 0.20 O2. Tracheal explants incubated for 6 days under 0.20 and 0.40 O2 had intact epithelial surface with abundant cilia, and no mucus present (scanning electron microscopy). Explants incubated under 0.60 O2 contained mucus globules by 3 days. Incubation in 0.95 O2 resulted in mucus globules within 3 days and epithelial sloughing, loss of cilia, and increased mucus globules within 6 days. Time and O2 dependent increases in nuclear atypia, leukocytic infiltration into submucosal area, and focal flattening of pseudostratified ciliated epithelium were observed (light microscopy). These changes progressed following 6 days' exposure to 0.95 O2 to complete loss of normal epithelium, with broken-up, epithelial cells along the tracheal lumen.

Original languageEnglish (US)
Pages (from-to)285-293
Number of pages9
JournalRespiration Physiology
Volume53
Issue number3
DOIs
StatePublished - Jan 1 1983

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Mucus
Cilia
Epithelium
Leucine
Cricetinae
Electron Scanning Microscopy
Microscopy
Epithelial Cells
Light

All Science Journal Classification (ASJC) codes

  • Physiology
  • Pulmonary and Respiratory Medicine

Cite this

Effects of elevated Po2 upon tracheal explants. / Barnes, S. D.; Agee, C. C.; Peace, R. J.; Leffler, Charles.

In: Respiration Physiology, Vol. 53, No. 3, 01.01.1983, p. 285-293.

Research output: Contribution to journalArticle

Barnes, S. D. ; Agee, C. C. ; Peace, R. J. ; Leffler, Charles. / Effects of elevated Po2 upon tracheal explants. In: Respiration Physiology. 1983 ; Vol. 53, No. 3. pp. 285-293.
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